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High-pH LC-MS/MS of Vitamins B1 & B6
Vitamins B1 and B6 are two water-soluble vitamins with clinical research interest. Chromatographically, these are very challenging analytes for a reversed phase separation as they have negative values for the octanol/water partition coefficients. Presented here is a simple method that does not involve derivatization or ion pairing reagents. The method involves whole blood extraction at lower pH, cost effective internal standards, no derivatization and a reversed phase LC/MS/MS compatible method for the analysis. The assay is evaluated for precision, accuracy, linear range, and the results meet acceptance criteria.
High-pH LC-MS/MS of Vitamins B1 & B6
Vitamins B1 and B6 are two water-soluble vitamins with clinical research interest. Chromatographically, these are very challenging analytes for a reversed phase separation as they have negative values for the octanol/water partition coefficients. Presented here is a simple method that does not involve derivatization or ion pairing reagents. The method involves whole blood extraction at lower pH, cost effective internal standards, no derivatization and a reversed phase LC/MS/MS compatible method for the analysis. The assay is evaluated for precision, accuracy, linear range, and the results meet acceptance criteria.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
High-pH LC-MS/MS of Vitamins B1 & B6
Vitamins B1 and B6 are two water-soluble vitamins with clinical research interest. Chromatographically, these are very challenging analytes for a reversed phase separation as they have negative values for the octanol/water partition coefficients. Presented here is a simple method that does not involve derivatization or ion pairing reagents. The method involves whole blood extraction at lower pH, cost effective internal standards, no derivatization and a reversed phase LC/MS/MS compatible method for the analysis. The assay is evaluated for precision, accuracy, linear range, and the results meet acceptance criteria.
| Target Industries: | Clinical Research |
| Techniques: | HPLC, HPLC Reversed Phase |
| Brands: | Gemini |
| Separation Modes: | Reversed Phase |
| Phases: | Gemini C18 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.