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Reference HPLC Analysis of NSAID Active Ingredient Nimesulide from a Topical Gel Formulation Using a Kinetex 2.6 µm Polar C18 Column
The active ingredient Nimesulide is a nonsteroidal anti-inflammatory drug (NSAID) which can be formulated into a gel or tablet form. Nimesulide presents a reversed phase analytical challenge because it’s found in a charged state under typical LC pH conditions. Because of the relative polarity of Nimesulide, several proposed reversed phase methods suggest the use of an ion-pairing agent. In this application, we investigated a non-ion-pairing reversed phase method that leveraged the unique dual selectivity of the Kinetex Polar C18 which combines a polar modified surface and the hydrophobic selectivity of a C18 stationary phase.
Reference HPLC Analysis of NSAID Active Ingredient Nimesulide from a Topical Gel Formulation Using a Kinetex 2.6 µm Polar C18 Column
The active ingredient Nimesulide is a nonsteroidal anti-inflammatory drug (NSAID) which can be formulated into a gel or tablet form. Nimesulide presents a reversed phase analytical challenge because it’s found in a charged state under typical LC pH conditions. Because of the relative polarity of Nimesulide, several proposed reversed phase methods suggest the use of an ion-pairing agent. In this application, we investigated a non-ion-pairing reversed phase method that leveraged the unique dual selectivity of the Kinetex Polar C18 which combines a polar modified surface and the hydrophobic selectivity of a C18 stationary phase.