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A Rapid, Automatable Solution for Phosphatidylethanol (PEth) LC-MS/MS Analysis in Whole Blood
Phosphatidylethanol (PEth) has emerged as an ethanol specific marker for alcohol consumption. PEths are phospholipids with very similar structures, which makes them challenging to separate chromatographically. In this fast analysis, a Kinetex Core-Shell Biphenyl LC column was used with a direct, in-plate phospholipid enrichment and cleanup for PEth from whole blood. The automatable LC-MS/MS workflow, including sample preparation and column selection, greatly simplifies the analysis while achieving excellent analytical performance.
A Rapid, Automatable Solution for Phosphatidylethanol (PEth) LC-MS/MS Analysis in Whole Blood
Phosphatidylethanol (PEth) has emerged as an ethanol specific marker for alcohol consumption. PEths are phospholipids with very similar structures, which makes them challenging to separate chromatographically. In this fast analysis, a Kinetex Core-Shell Biphenyl LC column was used with a direct, in-plate phospholipid enrichment and cleanup for PEth from whole blood. The automatable LC-MS/MS workflow, including sample preparation and column selection, greatly simplifies the analysis while achieving excellent analytical performance.