LC|Brands|Claricep Flash LC Column
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claricep-flash-lc-column

Claricep Flash Columns

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Seamlessly Upgrade to Claricep Flash Chromatography Columns

Available in two flash medias (Irregular and Spherical) with multiple chemistries optimized for resolution and high loading capacity.

Claricep Column

  • The deactivated silica surface promotes compound stability
  • Excellent peak shape and performance for both acidic and basic compounds
  • High quality and reproducibility
  • Wide range of selectivities
  • High pressure tolerance
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Overview

Flash Chemistries/Media

Claricep CM utilizes silica that is further purified with a proprietary acid washing process which results in even lower surface activity to result in an improved peak shape and resolution.

Consistent High Performance: High loading capacity (high surface area > 350m2/g) combined with good resolution for tight band definition (no tailing)
Wide Range of Selectivities: Variety of sorbents to meet any separation needs -(Silica (Si), C18, C8, AQ C18, Phenyl, Cyano (CN), Diol (OH), Amino (NH2) and HILIC (Urea) along with a large variety of cartridge sizes from 4 g to several kilograms allows for purification scale-up from milligrams to kilograms
High Pressure Tolerance: Exceptional durability to withstand high pressures due to closed-top spin-welded technology on standard hardware sizes that allows for pressure up to 12 bar (180 PSI)
Excellent Hardware: Universal luer fittings for compatibility with any flash system for great compound purity & recovery and 100% guaranteed leak-free.
High Quality: Consistent homogeneous packing allows for no channeling and creates reproducible high plate count (N).

Claricep Deactivated Silica vs Traditional Unmodified Silica

Comparative separations may not be representative of all applications.

Flash Conditions:

Dimension
4.6 x 150 mm
Flow Rate
1.8 mL/min
Temperature
30 °C
Detector:
1.8 mL/min
Mobile Phase
Dichloromethane/Methanol (98:2)
Injection Volume
5 μL
UV @ 254 nm
1.8 mL/min
HPLC TEST
Unmodified and Deactivated Silica were packed into individual stainless steel columns (4.6 x 150 mm) and then evaluated on a HPLC System

Sample

Catechol 100 μg/mL

Irregular Silica Comparison

Ultra pure packing which results in much less abnormal surface activity. This produces chromatograms with much sharper peaks

Aniline Peak Symmetry and Retention Test

Comparative separations may not be representative of all applications.

Flash Conditions:

Column
Claricep Irregular Silica CS (40-60 μm, 60 Å, 40 g)
Flow Rate
20 mL/min
Temperature
Ambient
Detector
UV @ 254 nm
Mobile Phase
Dichloromethane/Methanol (99:1)
Brand I
Flash Irregular Silica (40 g)
Retention Time

CLARICEP CS: 4.090 min

Brand I: 4.373 min

Sample

Aniline

Flash Conditions:

Column
Claricep Irregular Silica CS
(40-60 μm, 60 Å, 40 g)
Temperature
Ambient
Detector
UV @ 254 nm
Mobile Phase
Hexane/Ethylacetate (gradient)
Brand I
Flash Irregular Silica Column (40 g)
Retention Time
CLARICEP CS: 4.090 min, Brand I: 4.373 min

Sample

Phenyl acetone, 4-aminobenzoic acid

Spherical Silica Comparison

Regular, similar-sized and high quality particles can be packed in a column much more uniformly which reduces eddy flow currents and lateral diffusion. The result is greater peak symmetry and baseline resolution than is possible with irregular silica particles.

Claricep Spherical Silica 20-30 µm vs. Claricep Spherical Silica 20 µm

Flash Conditions:

Column A
Claricep Spherical Silica (20-35 μm, 100 Å, 12 g, 2 columns in tandem)
Column B
Claricep Spherical Silica (20 μm, 100 Å, 12 g, 2 columns in tandem
Flow Rate:
12 mL/min
Detector
UV @ 254/220 nm (ambient)
Mobile Phase:
A: Hexane, B: Ethanol
Gradient : Time (min)
% B
0
5
20
55

Samples:

0.2 mL

Screw-on Formats

Chemistry Options

3
Irrregular

CS Silica

CM Silica

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Spherical

C18

AQ

C8

Diol

C8

Phenyl

CN

NH2

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