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clarityspe-sample-preparation-products
Clarity SPE
15-Minute Cleanup from Biological Samples
The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.
- Works for desalting and clean up of biological samples
- Specially formulated buffer solutions available for optimal performance
- Quick protocols for rapid LC-MS/MS results
- No liquid-liquid extractions (LLE)
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Overview
Oligonucleotide Purification Products
Clarity OTX™ SPE
Prior to LC-MS analysis, therapeutic oligonucleotides must be isolated from interferences such as salts, sugars, large proteins, and genomic DNA. Using a mixed-mode solid phase extraction (SPE) sorbent in conjunction with carefully formulated buffers, Clarity OTX consistently delivers high recoveries.
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Clarity OTX Pro SPE
As the next-generation OTX platform, Clarity OTX PRO uses an enhanced mixed-mode SPE sorbent, optimized buffers, and well-characterized method-development ranges to isolate therapeutic oligos from salts, sugars, proteins, and genomic DNA.
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Clarity QSP™ SPE
Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.
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Clarity RP-Desalting™
Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.
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Material Characteristics
Clarity Products
Particle Support
Bonded Phase
Particle Shape/Size (μm)
Pore Size(Å)
Surface Area (m2/g)
Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145
Clarity BioSolutions Product Selection
Purification
Clarity Oligo-RP™ LC Columns
Clarity Oligo-MS™ LC Columns
Clarity Oligo-XT LC Columns
Clarity Oligo-XT LC Columns
Clarity OTX™ LC Columns
Primary Use
RP-LC-MS analysis with optimized
selectivity and sensitivity
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
biological samples for LC-MS bioanalysis
Oligo Length
≤ 60 mer
≤ 60 mer
≤ 40 mer
Recommended Mobile Phase
TEA / HFIP
TEA/HFIP/MeOH
N/A
Characterization / Analysis
Clarity QSP™
Clarity Oligo-RP™ LC Columns
Clarity Oligo-XT LC Columns
Clarity Oligo-XT LC Columns
Clarity RP-Desalting™
Primary Use
High-throughput, trityl-on RPC
purification
purification
RP-HPLC purification of failure
sequences from target sequences
sequences from target sequences
Quick removal or salt & excess reagent
Purities
>90%
>90%
~70%
Recoveries
~90%
~70%
~70%
Synthesis Scale Load
Up to 50 µmol
Up to 50 µmol
Up to 1 µmol
Oligo Types
DNA, RNA/RNAi, Thioates, Dye-labled, Modified
Documents
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