How Solvent Shielding Technology™ Improves Protein Precipitation

One of the defining features of the Impact protein precipitation plate is its exclusive Solvent Shielding Technology™. This innovative approach improves protein precipitation by creating a controlled environment where organic solvents such as acetonitrile or methanol efficiently precipitate and separate proteins from plasma or tissue homogenates.

By preventing moving samples from collection plate to collection plate, , this technology ensures minimal analyte loss, resulting in higher reproducibility and accuracy. Researchers benefit from faster workflows, consistent performance, and cleaner extracts ready for downstream LC-MS analysis. In short, Solvent Shielding Technology™ helps laboratories reduce variability while maximizing analyte recovery.

Key Features and Benefits of Impact Protein Precipitation Plates

The Impact protein precipitation plates are specifically designed to simplify sample preparation workflows. Below are the standout features and benefits that set them apart:

• Versatility: Compatible with both plasma and tissue homogenate samples.
• Solvent flexibility: Works effectively with acetonitrile or methanol for precipitation.
• Square-well plate format: Designed to fit standard vacuum manifolds and automated liquid handling systems.
• 2 mL well capacity: Accommodates both small and large sample volumes, giving flexibility for different workflows.
• High throughput: Enables parallel sample processing, reducing preparation time significantly.
• Improved accuracy: Cleaner extracts reduce matrix interferences, enhancing LC and LC-MS/MS sensitivity.
• Ease of integration: Compatible with manual and automated workflows for flexible use.
• Reliable reproducibility: Delivers consistent results across multiple wells and runs.

By combining efficiency, scalability, and performance, the Impact protein precipitation plates provide a cost-effective and time-saving solution for laboratories seeking reliable sample cleanup.

How to Use Impact Plates

Using Impact plates is simple and efficient, allowing researchers to streamline sample preparation while maintaining accuracy. A typical protocol involves the following steps:

• Sample loading: Add plasma or tissue homogenate samples into the wells of the Impact plate.
• Solvent addition: Introduce acetonitrile or methanol to initiate protein precipitation.
• Mixing: Vortex or gently agitate the plate to ensure thorough mixing of solvent and sample.
• 2 mL well capacity: Accommodates both small and large sample volumes, giving flexibility for different workflows.
• Filtration/collection: Apply vacuum, positive pressure or centrifugation to pass the precipitated mixture through the plate. Proteins are retained, while the clean filtrate containing analytes is collected.
• Analysis-ready sample: Collect the clarified extract, which can be directly injected into LC or LC-MS/MS systems.

This streamlined workflow minimizes manual handling and reduces sample preparation variability. By allowing multiple samples to be processed simultaneously, Impact plates are ideal for high-throughput laboratories working with demanding bioanalytical studies.

If you need to remove phospholipids in addition to proteins, upgrade to Phree™ Phospholipid Removal Plates. These provide a fast and effective solution for removing both proteins and phospholipids from plasma while maintaining excellent recovery of target analytes.