
The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Combined with being 100 % aqueous stable, the Kinetex Polar C18 is a unique column that combines performance and selectivity.
*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us for any questions and information about fitting compatibility and any other column internal diameters.
| Carbon Load 9 (effective), 9 (effective) % | 
| Particle Size 2.6, 3 | 
| Recommended Use Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100% aqueous stability | 
Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100 % aqueous stability.



| Columns:  Kinetex 2.6 µm Polar C18 AQUA 3 µm C18 | |
| Dimension: 150 x 4.6 mm | |
| Mobile Phase: A: 20 mM Potassium Phosphate (pH 1.5) B: Methanol | |
| Gradient: Time (min)            0 1 7 7.01 14 | % B           5 30 30 0 0 | 
| Flow Rate: 1.2 mL/min | |
| Temperature: 22 °C | |
| Injection Volume: 5 µL | |
| Detection: UV @ 210 nm | |
| Analytes: 1. Thiamine 2. Nicotinamide 3. Pyridoxal 4. Pyridoxine 5. Pantothenic Acid | |

| Columns:  Kinetex 2.6 µm C18 Fully Porous 1.9 µm C18 Kinetex 2.6 µm Polar C18 Kinetex 2.6 µm PS C18 | |
| Dimension: 100 x 2.1 mm  | |
| Mobile Phase: A:  Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid | |
| Gradient: Time (min)            0 4 4.1 7 | % B           3 100 3 3 | 
| Flow Rate: 0.5 mL/min | |
| Temperature: 25 °C | |
| Detection: MS/MS SCIEX® API 4000™ | |
| Sample: Acesulfame | |
| Column | Back-Pressure (bar) | RT (min) | Peak Height (cps) | Est. T0* (min) | 
|---|---|---|---|---|
| Kinetex 2.6 µm C18 | 252 | 0.67 | 1.8E+06 | 0.34 | 
| Fully Porous 1.9 µm C18 | 432 | 1.07 | 1.0E+06 | 0.46 | 
| Kinetex 2.6 µm Polar C18 | 237 | 1.45 | 2.1E+06 | 0.34 | 
| Kinetex 2.6 µm PS C18 | 241 | 1.85 | 2.2E+06 | 0.34 |