Rezex RHM-Monosaccharide H+ (8%) Ion Exclusion HPLC Columns
- 8% cross-linked hydrogen
- For use with monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts
- Versatile column, generally run with a mobile phase of deionized water
- USP L17
HPLC
80
294
rezex-hplc-column
rezex-rhm-monosaccharide-h
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Order
Overview
Cross Reference Guide
Phase
Description |
Applications |
Bio-Rad® Aminex®
Supelco® Supelcogel™
Waters® Sugar-Pak™
Transgenomic® CARBOSep™
Sepax® Carbomi®
RHM-Monosaccharide (L17 USP packing)*
8 % cross-linked HYDROGEN
– Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts
HPX-87H
125-0140
125-0140
Supelcogel
C-610H & H
C-610H & H
N/A
ICSep ION-300 |
Carbomix H |
Retention Times for Some Carbohydrates and Sugar Alcohols
| Counter Ion Analyte |
RHM H+
Adonitol (Ribitol)
11.11
D-Altrose
10.21
D-(-)-Arabinose
11.24
D-(+)-Cellobiose
| 8.02 |
D-(+)-Digitoxose
12.59
Dulcitol
10.71
Meso-Erythritol
12.14
D-(-)-Fructose
10.31
L-(-)-Fucose
11.65
D-(+)-Galactose
10.19
Gentiobiose
7.87
D-(+)-Glucose
| 9.62 |
Inositol
9.98 |
Isomaltose
| 8.02 |
Lactose
8.32
Lactulose
8.57
D-Lyxose
10.68
| Counter Ion Analyte |
RHM H+
D-Maltose
8.18
Maltotriose
7.51
Maltulose
8.27
D-Mannitol
| 10.59 |
D-(+)-Mannose
10.16
Melibiose
8.14
D-(+)-Melezitose
7.54*
D-(+)-Raffinose
7.88*
L-(+)-Rhamnose
10.90
D-(-)-Ribose
11.42
Salicin
14.98
D-Sorbitol
| 10.83 |
Stachyose
7.27 |
Sucrose
| 9.24* |
Trehalose
8.32
Xylitol
11.78
D-(+)-Xylose
10.24
Conditions:
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
RI @ 40 °C
Mobile Phase:
Water (degassed)
Select Applications on Rezex RHM Monosaccharide Columns
Alcohol
Columns:
Rezex RCM-Monosaccharide Aminex HPX-87C
Dimension:
300 x 7.8 mm
Part No.:
00H-0132-K0
Flow Rate:
0.6 mL/min
Temperature:
60 °C
Detection:
RI
Mobile Phase:
Water
Sample:
1. Methanol
2. Ethanol
3. Isopropanol
4. n-Propanol
5. sec-Butanol
6. n-Butanol
Applications (partial list)
Call us for assistance in selecting the right column for your needs!
Specifications and Operating Recommendations
RAM Carbohydrate
Part No.
00H-0132-K0 |
Ionic Form
Hydrogen
Standard Dimensions
300 x 7.8 mm
Matrix
Sulfonated Styrene Divinyl Benzene
Cross Linking
8 %
Particle Size (μm)
8
Min. Efficiency (p/m) based on last peak
35,000
Typical Pressure (psi @ Testing Flow Rate)
275
Max. Pressure (psi @ Max Flow Rate)
1,000
Max. Flow Rate (mL/min)
1.0
Max. Temperature (°C)
85
Typical Mobile Phase
Water
pH Range
1-8
Guard Column Part
03B-0132-K0
Cleaning, Regeneration and Storage
Organic Modifiers (Max)
5 % Methanol, IPA, EtOH
Inorganic Modifiers
5 % HNO3, H3PO4
Avoid
Acids, Bases, Salts or Metal Ions, > 30 % Acetonitrile
Cleaning Solvent
100 % Water
Flow Rate(mL/min)
0.4
Temperature (°C)
85
Duration (hrs)
12
Regeneration Solvent
0.025 M H2SO4
Flow Rate (mL/min)
0.2
Temperature (°C)
85
Duration (hrs)
4-16
Ship/Storage Solvent
Water