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Phase Detail
Rezex RHM-Monosaccharide H<sup>+</sup> (8%) Ion Exclusion HPLC Columns<br class="t-last-br" />
Rezex RHM-Monosaccharide H+ (8%) Ion exclusion columns are packed with an 8% cross-linked hydrogen ion exclusion media
Rezex RHM-Monosaccharide H+ (8%) Ion Exclusion HPLC Columns
Rezex RHM-Monosaccharide H+ (8%) Ion Exclusion HPLC Columns
- 8% cross-linked hydrogen
- For use with monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts
- Versatile column, generally run with a mobile phase of deionized water
- USP L17
Particle Size 8 |
Recommended Use Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts |
Cross Reference Guide
| Phase | Description | Applications | Bio-Rad® Aminex® | Supelco® Supelcogel™ | Waters® SugarPak™ | Transgenomic® CARBOSep™ | Sepax® Carbomi® |
|---|---|---|---|---|---|---|---|
| RHM-Monosaccharide (L17 USP packing)* | 8 % cross-linked HYDROGEN | – Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts | HPX-87H 125-0140 |
Supelcogel C-610H & H |
N/A | ICSep ION-300 |
Carbomix H |
Retention Times for Some Carbohydrates and Sugar Alcohols
| Counter Ion Analyte | RHM H+ |
|---|---|
| Adonitol (Ribitol) | 11.11 |
| D-Altrose | 10.21 |
| D-(-)-Arabinose | 11.24 |
| D-(+)-Cellobiose | 8.02 |
| D-(+)-Digitoxose | 12.59 |
| Dulcitol | 10.71 |
| Meso-Erythritol | 12.14 |
| D-(-)-Fructose | 10.31 |
| L-(-)-Fucose | 11.65 |
| D-(+)-Galactose | 10.19 |
| Gentiobiose | 7.87 |
| D-(+)-Glucose | 9.62 |
| Inositol | 9.98 |
| Isomaltose | 8.02 |
| Lactose | 8.32 |
| Lactulose | 8.57 |
| D-Lyxose | 10.68 |
Conditions:
Dimension:
300 x 7.8 mm |
Mobile Phase:
Water (degassed) |
Flow Rate:
0.6 mL/min |
Temperature:
80 °C |
Detection:
RI @ 40 °C |
| Counter Ion Analyte | RHM H+ |
|---|---|
| D-Maltose | 8.18 |
| Maltotriose | 7.51 |
| Maltulose | 8.27 |
| D-Mannitol | 10.59 |
| D-(+)-Mannose | 10.16 |
| Melibiose | 8.14 |
| D-(+)-Melezitose | 7.54* |
| D-(+)-Raffinose | 7.88* |
| L-(+)-Rhamnose | 10.90 |
| D-(-)-Ribose | 11.42 |
| Salicin | 14.98 |
| D-Sorbitol | 10.83 |
| Stachyose | 7.27 |
| Sucrose | 9.24* |
| Trehalose | 8.32 |
| Xylitol | 11.78 |
| D-(+)-Xylose | 10.24 |
* Partial hydrolysis results.
Select Applications on Rezex RHM Monosaccharide Columns
Alcohol
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Columns:
Rezex RCM-Monosaccharide Aminex HPX-87C
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Dimension:
300 x 7.8 mm
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Part No.:
00H-0132-K0
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Mobile Phase:
Water
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Flow Rate:
0.6 mL/min
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Temperature:
60 °C
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Detection:
RI
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Sample:
1. Methanol
2. Ethanol 3. Isopropanol 4. n-Propanol 5. sec-Butanol 6. n-Butanol |
Applications (partial list)
Call us for assistance in selecting the right column for your needs!
| Compound | App ID No. |
|---|---|
| Acetic Acid | 14862 |
| Citric Acid | 14970 |
| Formic Acid | 14962 |
| Lactic Acid | 14970, 14971 |
| Compound | App ID No. |
|---|---|
| Oxalic Acid | 14962 |
| Ribitol | 14991 |
| Succinic Acid | 14962 |
| Tartaric Acid | 14970 |
Specifications and Operating Recommendations
| Rezex RHM Monosaccharide |   |
|---|---|
| Part No. | 00H-0132-K0 |
| Ionic Form | Hydrogen |
| Standard Dimensions | 300 x 7.8 mm |
| Matrix | Sulfonated Styrene Divinyl Benzene |
| Cross Linking | 8 % |
| Particle Size (μm) | 8 |
| Min. Efficiency (p/m) based on last peak | 35,000 |
| Typical Pressure (psi @ Testing Flow Rate) | 275 |
| Max. Pressure (psi @ Max Flow Rate) | 1,000 |
| Max. Flow Rate (mL/min) | 1.0 |
| Max. Temperature (°C) | 85 |
| Typical Mobile Phase | Water |
| pH Range | 1-8 |
| Guard Column Part | 03B-0132-K0 |
| Cleaning, Regeneration and Storage |   |
|---|---|
| Organic Modifiers (Max) | 5 % Methanol, IPA, EtOH |
| Inorganic Modifiers | 5 % HNO3, H3PO4 |
| Avoid | Acids, Bases, Salts or Metal Ions, > 30 % Acetonitrile |
| Cleaning Solvent | 100 % Water |
| Flow Rate(mL/min) | 0.4 |
| Temperature (°C) | 85 |
| Duration (hrs) | 12 |
| Regeneration Solvent | 0.025 M H2SO4 |
| Flow Rate (mL/min) | 0.2 |
| Duration (hrs) | 4-16 |
| Ship/Storage Solvent | Water |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.