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Product Information
Phase Detail
Zebron ZB-BAC-1 GC Columns
Zebron ZB-BAC-1 GC columns is ideal for the resolution of ethanol and acetone

Zebron ZB-BAC-1 GC Columns

Fast, Accurate Blood Alcohol Analysis

  • Proprietary column set specially designed for accurate blood alcohol analysis — enhance resolution of ethanol and acetone peaks
  • Resolves t-butanol and n-propanol for greater selection of internal standards
  • Fast run time with baseline resolution of key components in just 2 minutes
  • Achieve dual column confirmation - get two elution order changes with ZB-BAC-2

Need an alternate selectivity for blood alcohol confirmation? See ZB-BAC-2.  
Testing drugs of abuse? See ZB-Drug-1.

Recommended Use
Abused Inhalant Anesthetics, Blood Alcohol Analysis


Agilent® Restek®
DB®-ALC1
DB-ALC2
Rtx®-BAC1
Rtx-BAC2
Rtx-VMS

*not exact equivalent, selectivity may differ


Faster, More Sensitive Blood Alcohol Analysis

Conditions for both columns:

Column:
As listed
Dimension:
As listed
Injection:
Split 0.8:1 @ 150 °C, 1 mL
Carrier Gas:
Helium @ 80 cm/sec (constant flow)
Oven Program:
40 °C (Isothermal)
Detector:
FID @ 250 °C
Sample:
Analytes 0.025 % and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)


Comparative separations may not be representative of all applications.
Conditions for both columns:

Column:
As listed
Dimension:
As listed
Part No.:
7HK-G021-36 (ZB-BAC-1)
7HK-G022-32 (ZB-BAC-2)
Injection:
Split 5:1 @ 150 °C, 1 mL
Carrier Gas:
Helium @ 80 cm/sec (constant flow)
Oven Program:
40 °C (Isothermal)
Detector:
FID @ 220 °C
Sample:
Analytes and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)

In contrast, the Phenomenex Zebron ZB-BAC-1 & and ZB-BAC-2 columns were able to fully resolve both t-Butanol and 2-Butanol from other target analytes, while maintaining rapid analysis times. Quantitative analysis on the Zebron BAC pair showed equivalent performance between all three internal standards (n-Propanol, t-Butanol, and 2-Butanol). The LOD & and LOQ for this column set were far below the control limits required by current testing regulations.

Estimated Limits of Detection (LOD) and Quantitation (LOQ) on Zebron ZB-BAC1 and ZB-BAC2 phases. The LODs and LOQs are also displayed in units of ppm in addition to % blood alcohol content.

Compound

Zebron ZB-BAC1

Zebron ZB-BAC2

LOD

LOQ

LOD

LOQ

Methanol

0.0003 %
(2.9 ppm)

0.001 %
(9.7 ppm)

0.0003 %
(3.0 ppm)

0.001 %
(10.1 ppm)

Acetaldehyde

0.00002 %
(0.2 ppm)

0.00008 %
(0.8 ppm)

0.00002 %
(0.2 ppm)

0.00006 %
(0.6 ppm)

Ethanol

0.0003 %
(2.6 ppm)

0.0009 %
(8.8 ppm)

0.0002 %
(2.2 ppm)

0.0007 %
(7.4 ppm)

Isopropanol

0.0002 %
(1.8 ppm)

0.0006 %
(5.8 ppm)

0.0001 %
(1.4 ppm)

0.0005 %
(4.6 ppm)

Acetone

0.00008 %
(0.8 ppm)

0.0003 %
(2.6 ppm)

0.00005 %
(0.5 ppm)

0.0002 %
(1.7 ppm)

High reproducibility of Zebron ZB-BAC1 and ZB-BAC2 for blood alcohols. Relative RSD values for replicate injections (n=5) were taken at the lowest concentration in the calibration curve of 0.025 %.


Zebron ZB-BAC-1

Compound
Peak Area % RSD (Absolute)
Peak Area % RSD (Relative to t-Butanol)
Peak Area % RSD (Relative to n-Propanol)
Peak Area % RSD (Relative to 2-Butanol)
Methanol
3.1
2.8
2.7
2.6
Acetaldehyde
2.1
1.6
1.5
1.4
Ethanol
3.4
2.8
2.7
2.6
Isopropanol
3.2
2.6
2.5
2.4
Acetone
2.2
1.6
1.5
1.4

Zebron ZB-BAC-2

Compound
Peak Area % RSD (Absolute)
Peak Area % RSD (Relative to t-Butanol)
Peak Area % RSD (Relative to n-Propanol)
Peak Area % RSD (Relative to 2-Butanol)
Methanol
2.1
1.6
1.4
1.3
Acetaldehyde
3.1
2.7
2.5
2.4
Ethanol
3.2
2.7
2.5
2.4
Isopropanol
2.2
1.6
1.4
1.3
Acetone
3.2
2.7
2.5
2.4

Upgrade to Zebron from these similar* phases: