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Product Information
Ion Exchange HPLC
Explore Phenomenex's ion exchange HPLC columns for efficient separation of charged analytes. Discover cation & anion exchange chromatography solutions.

Ion Exchange HPLC

Ion exchange (IEX) chromatography involves interactions between a charged stationary phase and the oppositely charged mobile analytes. In cation exchange chromatography positively charged molecules are attracted to a negatively charged stationary phase. Likewise, in anion-exchange chromatography negatively charged molecules are attracted to a positively charged stationary phase.

Ion exchange (IEX) chromatography is useful for a wide variety of compounds, from acidic (anionic) and basic (cationic) small molecules up to peptides and proteins.

Ion exchange columns are essential components in ion exchange chromatography, with cation exchange chromatography specifically focusing on the separation of positively charged molecules using columns with negatively charged functional groups. The selection of the appropriate column, buffer pH, and salt concentration are critical factors in the successful application of cation exchange chromatography.


Brand Description:
Luna SCX Strong selectivity for positively charged compounds
bioZen WCX Efficient weak cation exchange phase that allows you to separate proteins (especially monoclonal antibodies) from acidic/basic variants
PhenoSphere SAX Fully porous 80A strong anion-exchanger