Documents
Separation and Quantitation of Physiologic Cobalamins (Vitamin B12) in Dietary Supplements by LC-MS/MS using Synergi Fusion-RP Column (TN-1208)
Analysis of cobalamins (Vitamin B12 ) on reversed phase columns can be challenging due to the relative chemical instability of the analyte, the complexity of matrix effects and the existence of several vitamer forms of B12. In this tech note, we describe a selective, efficient and reproducible method to separate three B12 vitamers (methylcobalamin, cyanocobalamin and adenosylcobalamin) appropriate for the quantitative analysis of dietary supplements via LC-MS/MS.
Separation and Quantitation of Physiologic Cobalamins (Vitamin B12) in Dietary Supplements by LC-MS/MS using Synergi Fusion-RP Column (TN-1208)
Analysis of cobalamins (Vitamin B12 ) on reversed phase columns can be challenging due to the relative chemical instability of the analyte, the complexity of matrix effects and the existence of several vitamer forms of B12. In this tech note, we describe a selective, efficient and reproducible method to separate three B12 vitamers (methylcobalamin, cyanocobalamin and adenosylcobalamin) appropriate for the quantitative analysis of dietary supplements via LC-MS/MS.
Document Type:
Technical Notes
Target Industries:
Techniques:
Separation Modes:
Reversed Phase
Phases:
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