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Vitamin B12 in Supplements via LC-MS/MS
Analysis of cobalamins (Vitamin B12 ) on reversed phase columns can be challenging due to the relative chemical instability of the analyte, the complexity of matrix effects and the existence of several vitamer forms of B12. In this tech note, we describe a selective, efficient and reproducible method to separate three B12 vitamers (methylcobalamin, cyanocobalamin and adenosylcobalamin) appropriate for the quantitative analysis of dietary supplements via LC-MS/MS.
Vitamin B12 in Supplements via LC-MS/MS
Analysis of cobalamins (Vitamin B12 ) on reversed phase columns can be challenging due to the relative chemical instability of the analyte, the complexity of matrix effects and the existence of several vitamer forms of B12. In this tech note, we describe a selective, efficient and reproducible method to separate three B12 vitamers (methylcobalamin, cyanocobalamin and adenosylcobalamin) appropriate for the quantitative analysis of dietary supplements via LC-MS/MS.
Document Type:
Technical Notes
Target Industries:
Techniques:
Separation Modes:
Reversed Phase
Phases:
Vitamin B12 in Supplements via LC-MS/MS
Analysis of cobalamins (Vitamin B12 ) on reversed phase columns can be challenging due to the relative chemical instability of the analyte, the complexity of matrix effects and the existence of several vitamer forms of B12. In this tech note, we describe a selective, efficient and reproducible method to separate three B12 vitamers (methylcobalamin, cyanocobalamin and adenosylcobalamin) appropriate for the quantitative analysis of dietary supplements via LC-MS/MS.
| Target Industries: | Food Testing. Food Safety & Food Quality Analysis. |
| Techniques: | HPLC, HPLC Reversed Phase |
| Separation Modes: | Reversed Phase |
| Phases: | Synergi Fusion-RP |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.