USP Colchicine Assay on Luna 5 µm C8(2) and Kinetex 5 µm C8 (AN-1006)

This application note for the LC-UV assay for Colchicine is based on the official USP assay method which calls for an L7 (C8) 5 µm, 250 x 4.6 mm column under isocratic conditions. In this application note we utilized fully porous C8 and core shell C8 columns, Luna C8(2) and Kinetex C8, respectively. In addition, a shorter 150 mm Kinetex 5 µm C8 column was utilized to demonstrate an allowable adjustment per USP General Chapter <621>. All columns met the system suitability requirements for the assay.

Document Type:

Technical Notes

Target Industries:

Pharmaceutical

Techniques:

HPLC, HPLC Reversed Phase

Brands:

Kinetex, Luna HPLC Columns

Separation Modes:

Reversed Phase

Phases:

Kinetex C8, Luna C8(2)