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USP Colchicine Assay on Luna 5 µm C8(2) and Kinetex 5 µm C8 (AN-1006)
This application note for the LC-UV assay for Colchicine is based on the official USP assay method which calls for an L7 (C8) 5 µm, 250 x 4.6 mm column under isocratic conditions. In this application note we utilized fully porous C8 and core shell C8 columns, Luna C8(2) and Kinetex C8, respectively. In addition, a shorter 150 mm Kinetex 5 µm C8 column was utilized to demonstrate an allowable adjustment per USP General Chapter <621>. All columns met the system suitability requirements for the assay.
USP Colchicine Assay on Luna 5 µm C8(2) and Kinetex 5 µm C8 (AN-1006)
This application note for the LC-UV assay for Colchicine is based on the official USP assay method which calls for an L7 (C8) 5 µm, 250 x 4.6 mm column under isocratic conditions. In this application note we utilized fully porous C8 and core shell C8 columns, Luna C8(2) and Kinetex C8, respectively. In addition, a shorter 150 mm Kinetex 5 µm C8 column was utilized to demonstrate an allowable adjustment per USP General Chapter <621>. All columns met the system suitability requirements for the assay.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
USP Colchicine Assay on Luna 5 µm C8(2) and Kinetex 5 µm C8 (AN-1006)
This application note for the LC-UV assay for Colchicine is based on the official USP assay method which calls for an L7 (C8) 5 µm, 250 x 4.6 mm column under isocratic conditions. In this application note we utilized fully porous C8 and core shell C8 columns, Luna C8(2) and Kinetex C8, respectively. In addition, a shorter 150 mm Kinetex 5 µm C8 column was utilized to demonstrate an allowable adjustment per USP General Chapter <621>. All columns met the system suitability requirements for the assay.
| Target Industries: | Pharmaceutical |
| Techniques: | HPLC, HPLC Reversed Phase |
| Brands: | Kinetex, Luna HPLC Columns |
| Separation Modes: | Reversed Phase |
| Phases: | Kinetex C8, Luna C8(2) |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.