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Column Bed Stability During Routine Size Exclusion Chromatography
The primary failure mode for size exclusion columns is column voiding, wherein the column packed bed compresses and collapses. In this application note we explore column method robustness against column voiding during routine testing using a 4.6 mm internal diameter (ID) by 150 mm length column packed with sub-2 µm, 200 Å media and run with a viscous mobile phase containing 10 % isopropanol using a Biozen dSEC-2 Column.
Column Bed Stability During Routine Size Exclusion Chromatography
The primary failure mode for size exclusion columns is column voiding, wherein the column packed bed compresses and collapses. In this application note we explore column method robustness against column voiding during routine testing using a 4.6 mm internal diameter (ID) by 150 mm length column packed with sub-2 µm, 200 Å media and run with a viscous mobile phase containing 10 % isopropanol using a Biozen dSEC-2 Column.