Documents
Maximizing Recovery of 25-OH Vitamin D2 and D3 using Phospholipid Removal Plates and Core-Shell LC-MS/MS Analysis (TN-0070)
This technical note presents preliminary data showing that simultaneously removing proteins and phospholipids from plasma samples with a Phenomenex Phree Phospholipid Removal Plate showed a superior sample cleanup compared to a standard protein precipitation method. Quantification of 25-OH Vitamin D and detection of LLOQ calibrators ( 5 nmol/L) was easier compared to samples where protein precipitation alone had been performed. This work was performed by the Royal Liverpool and Broadgreen University Hospital and was presented at the British Mass Spectrometry Society (BMSS) 2014 Annual Meeting.
Maximizing Recovery of 25-OH Vitamin D2 and D3 using Phospholipid Removal Plates and Core-Shell LC-MS/MS Analysis (TN-0070)
This technical note presents preliminary data showing that simultaneously removing proteins and phospholipids from plasma samples with a Phenomenex Phree Phospholipid Removal Plate showed a superior sample cleanup compared to a standard protein precipitation method. Quantification of 25-OH Vitamin D and detection of LLOQ calibrators ( 5 nmol/L) was easier compared to samples where protein precipitation alone had been performed. This work was performed by the Royal Liverpool and Broadgreen University Hospital and was presented at the British Mass Spectrometry Society (BMSS) 2014 Annual Meeting.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
CONNECT WITH US
