search
Documents
N-Nitroso Desmethyl Doxepin Analysis using a SCIEX 5500+ System
Desmethyl Doxepin is a tricyclic antidepressant that acts as a norepinephrine reuptake inhibitor. It has been determined to be the major active metabolite of Doxepin and is more potent than Doxepin alone. Due to the fact that it is a secondary amine, it is possible that an N-nitroso Desmethyl Doxepin impurity (NDSRI – nitroso drug substance related impurity) can form during synthesis or storage. As NDSRIs have the potential to be carcinogenic it is important to monitor the active pharmaceutical compound or formulations for the presence of the NDSRI. In this technical note, a method is described where N-nitroso Desmethyl Doxepin is separated from the parent drug, allowing for sensitive detection by LC-MS/MS.
N-Nitroso Desmethyl Doxepin Analysis using a SCIEX 5500+ System
Desmethyl Doxepin is a tricyclic antidepressant that acts as a norepinephrine reuptake inhibitor. It has been determined to be the major active metabolite of Doxepin and is more potent than Doxepin alone. Due to the fact that it is a secondary amine, it is possible that an N-nitroso Desmethyl Doxepin impurity (NDSRI – nitroso drug substance related impurity) can form during synthesis or storage. As NDSRIs have the potential to be carcinogenic it is important to monitor the active pharmaceutical compound or formulations for the presence of the NDSRI. In this technical note, a method is described where N-nitroso Desmethyl Doxepin is separated from the parent drug, allowing for sensitive detection by LC-MS/MS.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
N-Nitroso Desmethyl Doxepin Analysis using a SCIEX 5500+ System
Desmethyl Doxepin is a tricyclic antidepressant that acts as a norepinephrine reuptake inhibitor. It has been determined to be the major active metabolite of Doxepin and is more potent than Doxepin alone. Due to the fact that it is a secondary amine, it is possible that an N-nitroso Desmethyl Doxepin impurity (NDSRI – nitroso drug substance related impurity) can form during synthesis or storage. As NDSRIs have the potential to be carcinogenic it is important to monitor the active pharmaceutical compound or formulations for the presence of the NDSRI. In this technical note, a method is described where N-nitroso Desmethyl Doxepin is separated from the parent drug, allowing for sensitive detection by LC-MS/MS.
| Target Industries: | Nitrosamine Analysis, Pharmaceutical |
| Techniques: | HPLC, HPLC Reversed Phase |
| Brands: | Kinetex |
| Separation Modes: | Reversed Phase |
| Phases: | Kinetex Biphenyl |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.