Kinetex Biphenyl Column for Aromatic Compounds

Order

Overview

Novel Selectivity For Tough Separations

Think high performance‚ enhanced retention‚ and the ability to go where a traditional C18 can’t. The Kinetex Biphenyl offers the high performance benefits of a core-shell particle with a unique stationary phase capable of becoming the go-to selectivity for reversed phase method development. Use Kinetex Biphenyl columns to get enhanced retention‚ higher sensitivity‚ and overall better results;
especially for aromatic compounds.

Aromatic Selectivity

Compounds with aromatic ring structures offer a specific type of selectivity associated with pi-pi bond interaction. The compound’s aromaticity provides pi electrons that have the potential to interact with pi bonds, which can be found on phenyl based stationary phases. This provides a unique, orthogonal selectivity compared to a traditional C18 phase.

Conditions for all columns:

Column:

Kinetex 1.7 µm Biphenyl

Dimension:

50 x 2.1 mm

Flow Rate:

0.5 mL/min

Temperature:

40 ˚C

Detection:

MS/MS (Sciex® API 4000™) (ambient)

Mobile Phase:

A: Water with 0.1 % Formic Acid, B: Acetonitrile with 0.1 % Formic Acid

Part No.:

00B-4628-AN

Injection Volume:

10 μL

Gradient : Time (min)

% B

0

5

4

100

5

100

7

5

Sample:

Drugs of Abuse

Increased Separation of Aromatic Compounds

Using a Biphenyl Phase

The elution order of analytes can change depending upon the predominate selectivity characteristics of a HPLC or UHPLC column and the utilized mobile phase. Therefore, by altering the stationary phase or mobile phase conditions, we can observe a unique elution order and extend retention of more polar analytes.