Product Information
Brand
BioSep GFC/SEC HPLC Columns
BioSep gel filtration (GFC/SEC) columns provide efficient separation for biomolecules such as proteins, peptides, immunoglobulins

BioSep GFC/SEC HPLC Columns

Aqueous Size Exclusion (SEC) / Gel Filtration (GFC) for Protein and Peptides

Proteins & Peptides Simplified

BioSep Gel Filtration (GFC/SEC) columns are used to analyze and/or characterize proteins, peptides, and other biomolecules, including antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting. BioSep columns offer many important benefits to keep your research, method development/validation, and ongoing size exclusion separations simple. For new UHPLC/HPLC GFC method development, turn to Biozen SEC columns.

  • High Efficiencies for Greater Resolution
  • Expanded Resolution Windows
  • Excellent Reproducibility
  • Highly Inert for Better Recovery and Quantitation

BioSep-SEC-S HPLC Columns
BioSep SEC Columns

Phases

Gel Filtration Chromatography (GFC)
SEC-s3000
Hydrophilic bonded silica with a pore size of 290Å
Recommended Use
Ideal for immunoglobulin aggregate applications, medium to large proteins, and serum proteins.
Official Method
L33
Particle Size
5
SEC-s4000
Hydrophilic bonded silica with a pore size of 500Å
Recommended Use
Ideal for large proteins and PEGylated IgG.
Official Method
-
Particle Size
5
SEC-s2000
Ideal for biogeneric aggregate applications, peptide therapeutics, small proteins, PEGylated peptides, and PEGylated small proteins.
Recommended Use
Hydrophilic bonded silica with a pore size of 145Å
Official Method
L33, L35
Particle Size
5

BioSep GFC/SEC Columns Applications

Showing 1-6 of 6 results
Compare BioSep-SEC-S3000(2) & Shodex Protein KW803, Hu Serum
Albumin and IgG (2:3) ±Arginine on BioSep3000 (2)
MW Calibration Proteins on BioSep-SEC-S4000
Low MW proteins and peptides on BioSep2000(2) non-denaturing condition
Hu Serum and HSA on BioSep3000 (2)
Follitropin Reference Standard on 5 µm Hydrophilic Silica