Strata SPE products are manufactured to bring you optimal quality, reproducibility, and performance. With over 20 years of experience, Strata SPE products provide clean extracts and high recoveries for a multitude of target analytes across diverse sample matrices.
SPE Mechanism |
Analyte Functional Group |
Sorbent Functional Group |
Strata Sorbent |
---|---|---|---|
Reversed Phase |
R ![]() ![]() hydrocarbon ![]() ![]() aromatic |
R ![]() ![]() ![]() hydrocarbon ![]() ![]() aromatic |
C18-E, C18-U, C8 C18-T PH, SDBL |
Ion-Exchange |
NR4+ strong RNH3+ weak RSO3- strong RCO2- weak |
-O2C—weak -O3S—strong +H3N—weak +R3N—strong |
WCX Screen-C, SCX NH2 Screen-A, SAX |
Sample Matrix | Sorbent Mass |
---|---|
Blood, serum, plasma | 50 mg sorbent per 250 µL |
Urine | 50 mg sorbent per 500 µL |
Filtered tissue homogenates | 100 mg sorbent per 100 mg tissue |
Environmental Samples | Sorbent Mass |
---|---|
Water (particulate-free) drinking | 500 mg/100 mL - 500 mL sample |
Water (particulate-laden) rivers, runoff, etc. | 1 g/100 mL - 500 mL sample |
Soil extracts | 1 g/100 g of soil extract |
Food/Plant Material | 1 g/ 100g sample |
Sorbent Mass | 10 mg | 50 mg | 100 mg | 150 mg | 200 mg | 500 mg | 1 g | 2 g | 5 g | 10 g |
---|---|---|---|---|---|---|---|---|---|---|
Practical Minimum Wash and Elution Volume 4 bed volumes |
60 µL | 300 µL | 600 µL | 900 µL | 1.2 mL | 3 mL | 6 mL | 12 mL | 30 mL | 60 mL |
Recommended Wash and Elution Volume 8 bed volumes |
120 µL | 600 µL | 1.2 mL | 1.8 mL | 2.4 mL | 6 mL | 12 mL | 24 mL | 60 mL | 120 mL |
Column:
1 mL Methanol |
Equilibrate:
1 mL DI Water |
Load:
Pretreated sample |
Wash:
1 mL 5% Methanol in DI Water, dry under
vacuum for 2-5 min |
Elute:
1 mL Methanol |