Biozen Nano HPLC Columns
Biozen Nano LC Columns, developed by Phenomenex, are specialized liquid chromatography columns designed for high-sensitivity biomolecule separations, particularly suited for omics applications such as proteomics and metabolomics. These columns leverage advanced particle technology and innovative hardware to deliver superior performance, reproducibility, and ease of use.
Zero Dead-Volume Nano LC Connections in a CLICK
Biozen Nano’s fully integrated SecurityLINK finger-tight fitting system simplifies your system connections while providing consistent performance through the Torque Limiting Technology that prevents over-tightening or under-tightening, making every connection leak-free.
- Integrated SecurityLINK fittings for easy installation and leak-free connections
- Maximized resolution for improved protein/peptide identification
- Increased robustness and sensitivity
Overview
Better Sensitivity and Selectivity with Biozen Nano LC Columns
What is Torque Limiting Technology?
Once the perfect connection has been made through finger-tightening, the SecurityLINK fitting offers a haptic “CLICK” to confirm that optimum torque has been reached. This ensures a consistent connection every time and prevents over or under-tightening that may cause column performance issues.
System Compatibility
Biozen Nano Columns with integrated SecurityLINK Fittings were designed to be compatible with the majority of systems that are available on the market today. Compatible with any System with 1/16 in. Ports.
3 Nano Chemistries
Excellent retention by combined positively charged surface ligand and C18 ligand.
Biozen Peptide XB-C18
Overall retention of both acidic and basic peptides through C18 stationary phase with di-isobutyl side chains.
Biozen Polar C18
Enhanced selectivity / retention for polar analytes without diminishing useful non-polar retention
Features of Nano LC Columns
Nano LC columns are defined by their small size, low flow rates, and high sensitivity, making them ideal for analyzing limited samples in omics, pharmaceutical, and environmental research. Their versatility in formats and stationary phases, combined with compatibility with MS and UHPLC, positions them as powerful tools for high-resolution separations.
- Inner Diameter: ≤100 µm (commonly 75 µm)
- Flow Rate: 100 nL/min – 1 µL/min
- Sensitivity: Up to 4000–5000× higher than standard HPLC
- Construction: Fused-silica capillaries, polyimide coating, integrated frits
- Stationary Phases: C18, phenyl, polymeric, core-shell, monolithic, pillar array
- Pressure/Temperature: Up to 15,000 psi, up to 45°C Formats: Packed, monolithic, open tubular, pillar array
- Compatibility: Nano LC-MS systems, various connector types
- Applications: Proteomics, biomarker discovery, biopharma, low-abundance analytes
- Solvent Consumption: Significantly reduced
Torque Limiting Technology provides a tactile “CLICK” at optimal torque to prevent over- or under-tightening
- SecurityLINK™ finger-tight fittings ensure zero dead-volume, leak-free connections
- Easy, consistent installation with integrated fittings
- Maximized resolution improves protein/peptide identification
- Enhanced sensitivity and selectivity
- Increased robustness for reliable LC performance
How do Biozen Nano LC columns work?
Biozen Nano LC columns work by leveraging advanced core-shell or thermally modified particles, specialized chemistries, and biocompatible titanium hardware to deliver high-efficiency, reproducible separations for omics analysis. The SecurityLINK™ fitting system ensures leak-free connections, while the column design minimizes secondary interactions and enhances sensitivity in nano-flow LC-MS workflows. These features make the columns ideal for challenging applications like proteomics, peptide mapping, and viral peptide detection.