HILIC HPLC

HILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.

HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention.