HILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.
HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention.
Brand | Description: |
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Luna Omega SUGAR | Polyamide/amine phase for the separation of polar analytes such as polyols/sugars |
Kinetex HILIC | Novel core-shell unbonded silica phase provides significant selectivity for retention and separation of polar compounds |
Luna HILIC | Offers excellent MS sensitivity and selectivity for polar compounds |
Luna NH2 | Amine phase applicable to multiple modes of chromatography and classes of polar compounds |
Luna Silica(2) | Ultra-pure, metal-free silica particle that offers great polar selectivity. |
bioZen Glycan | Higher order separations of released N-Glycans and biomarkers. |