|LC|Brands|Biozen Biologics LC Columns|Biozen Glycan|
HPLC
22
2348
biozenlc-hplc-column
biozen-glycan

Biozen Glycan

The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems, can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities, to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the Biozen Glycan excels with increased polar retention and selectivity.
#f2f5f9
smooth-scroll-vertical-tabs

Order

tabname
Order

Overview

Minimize your priming with our BioTi™ Biocompatible hardware

Biozen Titanium BioTi Hardware

Columns:
Biozen 1.8 μm SEC-3
Dimension:
150 x 4.6 mm
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0

Sample:

  1. γ-Globulin, 5 mg/mL
  2. Ovalbumin, 1 mg/mL

Conditions for both separations:

Column:
Biozen 2.6 µm Glycan
Dimension
150 x 2.1 mm
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection
FLD ex/em 285/345 nm
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5, B: Acetonitrile
Part No.:
00F-4773-AN
Gradient : Time (min)
% B
0
78
10
74.5
24
72
38.5
55.9
38.6
40
40.6
40
40.7
78
48
78

Sample:
As noted

Sample Preparation Solutions

N-Gycan Clean-Up HILIC Solid Phase Extraction (SPE)

High recovery of labeled, released N-glycans in a microelution 96-well plate format allowing for streamlined processing and clean-up of small sample volumes.

LEARN MORE

MagBeads Streptavidin Coated

Higher binding capacity magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

LEARN MORE

bioZen Glycan Batch-to-Batch Reproducibility

Conditions for all separations:

Column:
bioZen 2.6 μm Glycan
Dimension
100 x 2.1 mm
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection
FLD ex/em 330/420 nm
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5, B: Acetonitrile
Part No.:
00D-4773-AN
Gradient : Time (min)
% B
0
76
16
72
25.9
40
27.2
40
27.3
76
30
76

Sample:
Human IgG Glycan Library

2-AB Labeled Bovine Fetuine Sialylated Glycans

Column:
bioZen 2.6 μm Glycan
Dimension
100 x 2.1 mm
Flow Rate:
0.5 mL/min
Temperature:
80 °C
Detection
FLD ex/em 330/420 nm
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5, B: Acetonitrile
Part No.:
00F-4773-AN
Gradient : Time (min)
% B
0
60
20
20
24
20
24.1
60
30
60

Sample:
Bovine Fetuin

Glycan Analysis: HILIC

Column:
bioZen 2.6 μm Glycan
Dimension
100 x 2.1 mm
Flow Rate:
As Noted
Temperature:
50 °C
Detection
FLD ex/em 330/420 nm
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5, B: Acetonitrile
Part No.:
00F-4773-AN
Gradient : Time (min)
% B
0
76
16
72
25.9
40
27.2
40
27.3
76
30
76

Sample:
Human IgG Glycan Library

tabname
Overview
25942,25752,25436

Applications

View All Applications
/resources/application-library
tabname
Applications

Documents

/documents/biozen-solutions-flyer
/documents/biozen-phase-poster
/documents/biocompatible-hardware-brochure
/documents/biosimilars-chromatography-characterization-guide
/documents/biozen-column-care--use-manual
/documents/lc-msms-separation-of-38-amino-acids-in-hilic-mode
/documents/quantitation-of-neutral-and-sialylated-glycans-of-glycoproteins-using-biozen-hilic-chromatography-an
/documents/released-nglycan-analysis-of-infliximabdyyb-including-sialylated-glycans-using-a-biozen-26-m-glycan
/documents/sample-cleanup-after-nlinked-glycan-release-and-labeling-using-biozen-nglycan-cleanup-hilic-spe-micr
View All Documents
/resources/documents/library#f-phxbrands=BiozenLC-HPLC-column
tabname
Documents