For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Product Information
Technique
Normal Phase HPLC
Discover the fundamentals of Normal Phase Chromatography (NPC). Explore the recommended normal phase HPLC columns like Luna Silica, CN, NH2 & Kinetex HILIC.

Normal Phase HPLC

Normal phase chromatography is a type of chromatographic separation technique used in analytical chemistry to separate compounds based on their polarity. In normal-phase chromatography, the stationary phase is polar, while the mobile phase is non-polar. This means that compounds with higher polarity tend to interact more strongly with the stationary phase, causing them to move more slowly through the column, while less polar compounds move more quickly.

Normal phase chromatography (NPC) uses normal-phase HPLC columns to separate hydrophobic compounds and matrices that are retained too strongly by reversed phase and have minimal solubility in aqueous mobile phases. Stationary phases in normal-phase liquid chromatography typically include polar functional groups (silica, amino, and cyano) that interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.

hplc-silica

Brand Description:
Luna Silica(2) Recommended for L3 USP methods
Luna CN Recommended for L10 USP methods
Luna NH2 Recommended for L8 USP Method
Kinetex HILIC Core-Shell Unbonded Silica