For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Product Information
Technique
Normal Phase HPLC
Normal phase chromatography is used for compounds that are retained too tightly by reversed phase

Normal Phase HPLC

Normal phase chromatography (NPC) is used to separate hydrophobic compounds and matrices that are retained too strongly by reversed phase and have minimal solubility in aqueous mobile phases. Normal phase stationary phases typically include polar functional groups that interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.

Normal phase stationary phases typically include polar functional groups (silica, amino, and cyano) and interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.

Bare silica used for Normal Phase

Brand Description:
Luna Silica(2) Recommended for L3 USP methods
Luna CN Recommended for L10 USP methods
Luna NH2 Recommended for L8 USP Method
Kinetex HILIC Core-Shell Unbonded Silica