Normal Phase HPLC

Normal phase chromatography (NPC) is used to separate hydrophobic compounds and matrices that are retained too strongly by reversed phase and have minimal solubility in aqueous mobile phases. Normal phase stationary phases typically include polar functional groups that interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.

Normal phase stationary phases typically include polar functional groups (silica, amino, and cyano) and interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.