Optimizing Separation for Charge Variant Analysis (CVA) using Biozen 6 μm WCX: Salt versus pH Gradients (TN-1245)

An ion-exchange column, Biozen WCX, was recently introduced and complements the portfolio of Biozen products for biomolecule analysis. Biozen WCX is significantly more efficient than other WCX columns on the market and couples best in class particle technology with inert BioTi hardware to give a complete solution. Performing method development for charge variant analysis (CVA) using Biozen WCX columns will be discussed and the benefits of each elution method. The differences in pH and salt gradients for CVA will also be discussed.