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Beta-Glucuronidase Removal from Hydrolyzed Pain Management Urine Samples (TN-0083)
The escalating abuse of pain medicines has mandated that health care providers and practitioners routinely perform urine drug testing. Most laboratories opt for a dilute-and-shoot approach, along with enzymatic hydrolysis that requires no sample cleanup. The addition of extra protein as a result of hydrolysis using ß-glucuronidase to the urine can result in fouling of the LC column and loss of productivity. In this work, we present an automated method that uses an Impact Protein Precipitation 96-well plate to remove a majority of the proteins from hydrolyzed urine.
Beta-Glucuronidase Removal from Hydrolyzed Pain Management Urine Samples (TN-0083)
The escalating abuse of pain medicines has mandated that health care providers and practitioners routinely perform urine drug testing. Most laboratories opt for a dilute-and-shoot approach, along with enzymatic hydrolysis that requires no sample cleanup. The addition of extra protein as a result of hydrolysis using ß-glucuronidase to the urine can result in fouling of the LC column and loss of productivity. In this work, we present an automated method that uses an Impact Protein Precipitation 96-well plate to remove a majority of the proteins from hydrolyzed urine.
Document Type:
Technical Notes
Techniques:
Separation Modes:
Reversed Phase
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