Optimized Column Selectivity for Orthogonal Separation of Fatty Acid Methyl Esters (FAMEs) Using GCxGC (TN-2064)

An advanced version of GC is GCxGC, which employs two GC columns of complementary selectivity. The two columns are placed in two separate GC ovens and are connected in series to enhance GC separation power. A cryo-focusing modulator serves as the heart of GCxGC separations; it slices the peak produced by the first column and focuses it on the head of the second column, which provides a comprehensive separation of analytes in both dimensions. The best separation power is realized if the columns offer complementary selectivity. Presented here is an analysis of FAMEs, where a Zebron ZB-FAME column is used as a first dimension and a Zebron ZB- 5MSPLUS column is used as the second dimension.