Comparison of the Reversed Phase Retention of Six Extremely Polar Artificial Sweeteners with two polar modified Kinetex Core-shell C18 Columns with MS/MS Detection

In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners. The analysis of extremely polar compounds presents a unique reversed phase method development challenge due to a lack of polar selectivity and related peak shape issues. This can be mitigated by the incorporation of additives to the mobile phase such as ionic pairing reagents to increase both retention and selectivity, as well as improve peak shape.