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Low Molecular Weight Separation by GFC (TN-1083)
To obtain the optimal resolution of low molecular weight peptides and proteins by Gel Filtration Chromatography (GFC), one must use unique mobile phase conditions compared to the near-physiological conditions used for protein separation. For peptides, organic solvents and ion-pairing reagent (TFA) are used to minimize interactions resulting in sharp peaks with distinct retention typically based on the molecular weight of the peptide on a BioSep-SEC-S2000 GFC column
Low Molecular Weight Separation by GFC (TN-1083)
To obtain the optimal resolution of low molecular weight peptides and proteins by Gel Filtration Chromatography (GFC), one must use unique mobile phase conditions compared to the near-physiological conditions used for protein separation. For peptides, organic solvents and ion-pairing reagent (TFA) are used to minimize interactions resulting in sharp peaks with distinct retention typically based on the molecular weight of the peptide on a BioSep-SEC-S2000 GFC column
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Size Exclusion
Low Molecular Weight Separation by GFC (TN-1083)
To obtain the optimal resolution of low molecular weight peptides and proteins by Gel Filtration Chromatography (GFC), one must use unique mobile phase conditions compared to the near-physiological conditions used for protein separation. For peptides, organic solvents and ion-pairing reagent (TFA) are used to minimize interactions resulting in sharp peaks with distinct retention typically based on the molecular weight of the peptide on a BioSep-SEC-S2000 GFC column
| Target Industries: | Pharmaceutical |
| Techniques: | HPLC |
| Brands: | BioSep GFC/SEC Columns |
| Separation Modes: | Size Exclusion |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide