Loading Capacity of Biozen dSEC-2 Column Under SEC-HRMS Conditions

In this application note, the use of resistor tubing and 200 mM Ammonium Acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 µm dSEC 2 column . We tested the ability of increased sensitivity to yield accurate results with low sample loads, a common necessity. Some low abundance variants were below the limit of detection in the lower sample load injections. However, when the injection quantity exceeded the limit of detection for lower abundance variants, minor secondary exclusions began to appear.