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Loading Capacity of Biozen dSEC-2 Column Under SEC-HRMS Conditions
In this application note, the use of resistor tubing and 200 mM
Ammonium Acetate in the mobile phase was implemented to
measure loading capacity of a Biozen 1.8 µm dSEC 2 column .
We tested the ability of increased sensitivity to yield accurate
results with low sample loads, a common necessity. Some low
abundance variants were below the limit of detection in the lower
sample load injections. However, when the injection quantity
exceeded the limit of detection for lower abundance variants,
minor secondary exclusions began to appear.
Loading Capacity of Biozen dSEC-2 Column Under SEC-HRMS Conditions
In this application note, the use of resistor tubing and 200 mM
Ammonium Acetate in the mobile phase was implemented to
measure loading capacity of a Biozen 1.8 µm dSEC 2 column .
We tested the ability of increased sensitivity to yield accurate
results with low sample loads, a common necessity. Some low
abundance variants were below the limit of detection in the lower
sample load injections. However, when the injection quantity
exceeded the limit of detection for lower abundance variants,
minor secondary exclusions began to appear.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Size Exclusion
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