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Clarity HPLC/PREP Columns

Product Information
Brand
Clarity LC reversed phase HPLC columns provide effective purification for synthetic oligonucleotides. Clarity HPLC columns are available in C18 selectivities and core-shell particles for optimized efficiency.

 

Each product in Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.

  • Easily separate N-1 failure sequences from target oligo with > 90% purities
  • Trityl-off purification of DNA, RNA, Thioates, and modified/labeled oligonucleotides
  • Preparative dimensions and particle sizes for loads > 5μmol
  • Purify oligos up to 60 mer in length
  • Excellent column for reversed phase HPLC quality control (QC) testing

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LC-MS Analysis

Clarity Oligo-XT

Clarity Oligo-XT

  • First choice for analytical to preparative scale chromatography
  • pH stable 1-12
  • 100% aqueous mobile phase stable
  • Core-shell particle technology provides improved speed, resolution, and sensitivity
  • Easily scale analytical LC-MS methods to preparative scale for isolation/purification
  • 1.7µm particles offer increased performance over existing sub-2µm methods

 

Clarity Oligo-MS

Clarity Oligo-MS

  • Select if alternative selectivity is needed for analytical scale chromatography
  • pH stable from 1.5-8.5
  • Core-shell particle technology provides improved speed, resolution, and sensitivity
  • Easily transfer quantitative LC-MS methods to any system with 2.6µm particles
  • 1.7µm particles offer increased performance over existing sub-2µm methods

 

Clarity Oligo-RP

Clarity Oligo-RP

  • Select if alternative selectivity is needed for analytical to preparative scale chromatography
  • pH stable from 1.0-12
  • Silica-organic grafting to create composite particle for improved robustness
  • Easily scale analytical LC-MS methods to preparative scale for isolation/purification

 

Material Characteristics

Clarity Products Porous Particle Support Bonded Phase Particle Shape/Size (μm) Pore Size(Å) Surface Area (m2/g)

Clarity Oligo-XT LC Columns

Core-shell

C18

1.7, 2.6, 5

100

200

Clarity Oligo-MS LC Columns

Core-shell

C18

1.7, 2.6, 5

100

200

Clarity Oligo-RP LC Columns

TWIN (silica, organic composite) C18 3, 5, 10 110 375

C18

3, 5, 10

110

375

Clarity BioSolutions Product Selection

Purification

  Clarity QSP™ Clarity Oligo-RP™ LC Columns
Clarity Oligo-XT LC Columns
Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
purification
RP-HPLC purification of failure
sequences from target sequences
Quick removal or salt & excess reagent
Purities >90% >90% ~70%
Recoveries ~90% ~70% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

  Clarity Oligo-RP™ LC Columns Clarity Oligo-MS™ LC Columns
Clarity Oligo-XT LC Columns
Clarity OTX™ LC Columns
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A