Clarity HPLC/PREP Columns

Each product in Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.

Features of Clarity HPLC Columns for Oligonucleotides

Easily separate N-1 failure sequences from target oligo with > 90% purities
Trityl-off purification of DNA, RNA, Thioates, and modified/labeled oligonucleotides
Preparative dimensions and particle sizes for loads > 5μmol
Purify oligos up to 60 mer in length
Excellent column for reverse phase HPLC quality control (QC) testing

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LC-MS Analysis

Clarity Oligo-XT

First choice for analytical to preparative scale chromatography
pH stable 1-12
100% aqueous mobile phase stable
Core-shell particle technology provides improved speed, resolution, and sensitivity
Easily scale analytical LC-MS methods to preparative scale for isolation/purification
1.7µm particles offer increased performance over existing sub-2µm methods

Clarity Oligo-MS

Select if alternative selectivity is needed for analytical scale chromatography
pH stable from 1.5-8.5
Core-shell particle technology provides improved speed, resolution, and sensitivity
Easily transfer quantitative LC-MS methods to any system with 2.6µm particles
1.7µm particles offer increased performance over existing sub-2µm methods

Clarity Oligo-RP

Select if alternative selectivity is needed for analytical to preparative scale chromatography
pH stable from 1.0-12
Silica-organic grafting to create composite particle for improved robustness
Easily scale analytical LC-MS methods to preparative scale for isolation/purification

Material Characteristics

Clarity Products Porous	Particle Support	Bonded Phase	Particle Shape/Size (μm)	Pore Size(Å)	Surface Area (m²/g)
Clarity Oligo-XT LC Columns	Core-shell	C18	1.7, 2.6, 5	100	200
Clarity Oligo-MS LC Columns	Core-shell	C18	1.7, 2.6, 5	100	200
Clarity Oligo-RP LC Columns	TWIN (silica, organic composite) C18 3, 5, 10 110 375	C18	3, 5, 10	110	375

Clarity BioSolutions Product Selection

Purification

	Clarity QSP™	Clarity Oligo-RP™ LC Columns Clarity Oligo-XT LC Columns	Clarity RP-Desalting™
Primary Use	High-throughput, trityl-on RPC purification	RP-HPLC purification of failure sequences from target sequences	Quick removal or salt & excess reagent
Purities	>90%	>90%	~70%
Recoveries	~90%	~70%	~70%
Synthesis Scale Load	Up to 50 µmol	Up to 50 µmol	Up to 1 µmol
Oligo Types	DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

	Clarity Oligo-RP™ LC Columns	Clarity Oligo-MS™ LC Columns Clarity Oligo-XT LC Columns	Clarity OTX™ LC Columns
Primary Use	RP-LC-MS analysis with optimized selectivity and sensitivity	Rapid, high efficiency RP-LC-MS analysis for QC and characterization	Extraction of oligo therapeutics from biological samples for LC-MS bioanalysis
Oligo Length	≤ 60 mer	≤ 60 mer	≤ 40 mer
Recommended Mobile Phase	TEA / HFIP	TEA/HFIP/MeOH	N/A

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