Product Information
Brand
Clarity HPLC/PREP Columns
Clarity LC reversed phase HPLC columns provide effective purification for synthetic oligonucleotides. Clarity HPLC columns are available in C18 selectivities and core-shell particles for optimized efficiency.
Clarity HPLC/PREP Columns
Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.
- RPC, HPLC, PREP LC, desalting, and extraction solutions
- DNA, RNA/RNAi, longmers, dye-labeled, and modified oligonucleotides
- High efficiency LC-MS protocols for characterization/QC
- Personalized technical support and customer service
LC-MS Analysis test
Oligo-XT
An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times as compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results.
Clarity Core-Shell
Conventional Fully Porous
The Core-Shell Advantage
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.
| Fully Porous |
Clarity Core-Shell |
Average Efficiency Gain with Clarity* |
|
|---|---|---|---|
 |
VS. |  |
90 % Higher |
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VS. |  |
85 % Higher |
 |
VS. |  |
20 % Higher |
Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
*May not be representative of all applications
Analytical LC abc
Clarity Oligo-RPâ„¢
Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 µm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos.
A Highly Selective Analysis
Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.
Separation of failure N-1 from target N sequence
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Column: Clarity 3 µm Oligo-RP C18
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Dimensions: 50 x 4.6 mm
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Part No.: 00B-4441-E0
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Mobile Phase:
A: 50 mM TEAA pH 7.5 B: Methanol |
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Gradient: 10 % to 45 % B in 30 minutes
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Flow Rate: 1 mL/ min
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Detection: UV @ 260 nm
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Sample:
1. 40nt DNA with sequence
CTTCTGAACAGTTGATCTATG- CACTTCAGACTTATGATCA (2.5 μg) 2. 39nt DNA with sequence
TTCTGAACAGTTGATCTATGCAC- TTCAGACTTATGATCA (2.5 μg) |
Purification
DNA Purification
Clarity Oligo-RP columns allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.
Easily Scale from HPLC to Preparative Purification
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Column:
Clarity 3 µm Oligo-RP C18
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Dimensions:
A: 50 x 10.0 mm
B: 50 x 4.6 mm |
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Part No.:
A: 00B-4441-N0
B: 00B-4441-E0 |
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Mobile Phase:
A: 50 mM TEAA pH 7.5
B: Methanol |
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Gradient:
10 % to 60 % B in 20 minutes
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Flow Rate:
A: 4.7 mL/ min
B: 1.0 mL/ min |
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Detection:
UV @ 260 nm
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Sample:
20nt DNA
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