Documents
Improving Resolution for Size Exclusion Chromatography Methods by Optimization of Linear Velocity
Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is a unique separation modality in that lower flow rates often yield improvements in separation and resolution can improve quite considerably simply by running at a lower linear velocity. This application note demonstrates the utility of method improvements by running at a lower flow rate using the Biozen dSEC-2 SEC column for the aggregate analysis of NIST mAb, a common standard protein.
Improving Resolution for Size Exclusion Chromatography Methods by Optimization of Linear Velocity
Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is a unique separation modality in that lower flow rates often yield improvements in separation and resolution can improve quite considerably simply by running at a lower linear velocity. This application note demonstrates the utility of method improvements by running at a lower flow rate using the Biozen dSEC-2 SEC column for the aggregate analysis of NIST mAb, a common standard protein.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Size Exclusion
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