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Method Optimization for Purity Analysis of IgG Isotypes and F-Fusion Proteins by Intact Reversed Phase
Purity analysis by reversed phase LC is a primary method for the analysis of recombinant proteins. Fc-fusions and other monoclonal antibody isotypes may behave quite differently chromatographically. However, by simply adjusting gradient programs to optimize capacity factor or retention time, one can develop a streamlined, “platform” method for purity analysis that may be optimized depending on method requirements.
Method Optimization for Purity Analysis of IgG Isotypes and F-Fusion Proteins by Intact Reversed Phase
Purity analysis by reversed phase LC is a primary method for the analysis of recombinant proteins. Fc-fusions and other monoclonal antibody isotypes may behave quite differently chromatographically. However, by simply adjusting gradient programs to optimize capacity factor or retention time, one can develop a streamlined, “platform” method for purity analysis that may be optimized depending on method requirements.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
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