Liquid Chromatography Separation Modes
Chromatography is widely used because it can separate many different analytes. These analytes can be separated based on various molecular characteristics, as demonstrated by the separation modes listed here.
Reversed Phase
Non-Polar stationary phases used with polar mobile phase to separate hydrophobic compounds differing in the type and number of polar groups they contain.
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Chiral
Separate enantiomers (identical mirror image) through the use of a chiral stationary phase.
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Gel Filtration (GFC)
Biomolecules or polar polymers can be separated based on their molecular weight (stokes radii).
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Gel Permeation (GPC)
Efficient separations of analytes (typically ≥3 µm designed for system limitations of ≤6000 psi).
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HILIC
Retain and separate highly polar analytes which cannot be retained using reversed phase.
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Ion Exchange
Separates analytes based on net charge using functionally charged stationary phases.
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Ion Exclusion
Sulfonated SDVB polymers provide an environment to separate sugars, sugar alcohols and organic acids.
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Normal Phase
Polar stationary phases used with non-polar mobile phase to separate hydrophobic compounds differing in the type and number of polar groups they contain.
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