Strata-X Solid Phase Extraction
Robust, Accurate Polymeric Solid Phase Extraction (SPE) Chromatography Products
Capitalize on cleanliness without sacrificing recovery with Strata-X polymeric SPE sorbents for solid phase extraction procedure. Strata-X polymeric sorbents are available in unique selectivities that have been developed to cover a diverse spectrum of analytes and simplify the method development process for fast and efficient sample preparation.
- Tightly retain analytes
- Remove unwanted contaminants including phospholipids, proteins, and particulates for superior SPE chromatography
- Easily cleanup large or small volumes
- Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa
- The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes
Axia Preparative LC Columns
Guaranteed, Longest Lasting Preparative HPLC and SFC Column
Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative HPLC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.
Order
Product Finder
Explore the Best Phases for Your Separation Needs
Overview
Available Formats
The Complete Guide to Solid Phase Extraction (SPE)
Learn how to develop a customized method for SPE and view applications that are applicable to a variety of different industries. Download the Complete Guide to Solid Phase Extraction (SPE) to quickly develop a new method with application examples. Learn about this targeted form of Sample Preparation that allows you to isolate your analyte of interests while removing any interfering compounds that may be in your sample.
- Ultra Clean Extracts
- Concentration of samples for better chromatographic results
- Solvent switching got GC or LC compatibility
- Longer column lifetime and improved chromatographic results
Loading Matrices
Strata-X sample preparation offers a versatile and comprehensive solution for a wide array of matrices across various scientific and analytical domains. Its adaptable design and efficient sorbent technology make it suitable for diverse sample types, including complex biological samples, environmental samples, food and beverage matrices, and pharmaceutical formulations.
Polymeric SPE Options
The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.
Select Phase Based on Target Analyte
(pKa < 2)
Reversed Phase & Weak Anion-Exchange
Strata-X-AW
Di-amino
(pKa 2-4)
Reversed Phase & Strong Anion-Exchange
Strata-X-A
Quaternary Amine
(pKa 8-10)
Reversed Phase & Strong Cation-Exchange
Strata-X-C
Sulfonic Acid
(pKa 8-10)
Reversed Phase & Strong Cation-Exchange
Strata-X-C
Sulfonic Acid
Reversed Phase
Strata-X
N-Vinylpyrrolidone
Pharmaceutical: Compounds from Plasma
Improved Clean Up and Recovery of Pharmaceutical Compounds From Plasma: SPE vs. Liquid-liquid Extraction
B: 0.1 % Formic acid in Methanol
Gradient:
SPE Protocol
% Absolute Recovery for Diclofenac
Diclofenac spiked plasma sample (50 μg/mL) after extraction with Strata™-X. Flurbiprofen (IS) was added post-extraction at a concentration of 160 μg/mL. Note: the flurbiprofen was added post blow down, which is also post-extraction.
Recovery data for Amitriptyline resulting from SPE phase screening
Recovery of Metoprolol
Representative chromatogram of the neat standard at 25 ng/mL
EPA 552.1 by Strata-X-A on a ZB-XLB-HT
GC Conditions (App ID: 19322)
Column 30 m x 0.32 mm x 0.25 µm, Ea
Sample Notes:
Derivatization: Add 1mL of MTBE to the eluent in a screw cap vial or test tube. Heat the sample at 50ºC for 1.5 hours. Remove and allow the sample to cool to room temperature. Slowly add 10mL of saturated sodium bicarbonate and invert the sample several times releasing carbon dioxide gas frequently. Aspirate 100µL of the organic layer and transfer to a 2mL vial with insert. Add 10µL of internal standard (1,2,3-Trichloropropane) and inject into GC-ECD system.