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Size Exclusion Chromatographic (SEC) Method for Oligonucleotides Using Heated Columns
This study explores size exclusion chromatography (SEC) as an alternative to ion-pairing reverse-phase chromatography (IPRP) for oligonucleotide characterization. SEC offers high-efficiency separations with reduced sequence dependence, especially when using Biozen dSEC-2 and dSEC-7 columns at elevated temperatures. Low-salt mobile phases enable direct mass spectrometry interfacing for enhanced identification. Initial stability studies show strong reproducibility in peak area recovery and retention time. SEC provides a complementary approach to IPRP, improving analytical method development for oligonucleotide and mRNA analysis.
Size Exclusion Chromatographic (SEC) Method for Oligonucleotides Using Heated Columns
This study explores size exclusion chromatography (SEC) as an alternative to ion-pairing reverse-phase chromatography (IPRP) for oligonucleotide characterization. SEC offers high-efficiency separations with reduced sequence dependence, especially when using Biozen dSEC-2 and dSEC-7 columns at elevated temperatures. Low-salt mobile phases enable direct mass spectrometry interfacing for enhanced identification. Initial stability studies show strong reproducibility in peak area recovery and retention time. SEC provides a complementary approach to IPRP, improving analytical method development for oligonucleotide and mRNA analysis.
Document Type:
Poster
Target Industries:
Techniques:
Brands:
Separation Modes:
Size Exclusion
Phases:
Size Exclusion Chromatographic (SEC) Method for Oligonucleotides Using Heated Columns
This study explores size exclusion chromatography (SEC) as an alternative to ion-pairing reverse-phase chromatography (IPRP) for oligonucleotide characterization. SEC offers high-efficiency separations with reduced sequence dependence, especially when using Biozen dSEC-2 and dSEC-7 columns at elevated temperatures. Low-salt mobile phases enable direct mass spectrometry interfacing for enhanced identification. Initial stability studies show strong reproducibility in peak area recovery and retention time. SEC provides a complementary approach to IPRP, improving analytical method development for oligonucleotide and mRNA analysis.
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC-SEC |
| Brands: | Biozen LC |
| Separation Modes: | Size Exclusion |
| Phases: | Biozen dSEC-7 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.