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Hybrid-LCMS/MS Method for the Quantification of Enhertu in Rat Plasma Using Biozen Streptavidin Magbeads
Antibody drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu (trastuzumab deruxtecan) as a model, we developed an LC-MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6-600 nM, 75%-90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
Hybrid-LCMS/MS Method for the Quantification of Enhertu in Rat Plasma Using Biozen Streptavidin Magbeads
Antibody drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu (trastuzumab deruxtecan) as a model, we developed an LC-MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6-600 nM, 75%-90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
Hybrid-LCMS/MS Method for the Quantification of Enhertu in Rat Plasma Using Biozen Streptavidin Magbeads
Antibody drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu (trastuzumab deruxtecan) as a model, we developed an LC-MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6-600 nM, 75%-90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC, HPLC Reversed Phase, Immunocapture |
| Brands: | Biozen LC |
| Separation Modes: | Reversed Phase |
| Phases: | Biozen-Peptide-XB-C18 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.