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Hybrid-LCMS/MS Method for the Quantification of Enhertu� in Rat Plasma Using Biozen� Streptavidin Magbeads
Antibody�drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu� (trastuzumab deruxtecan) as a model, we developed an LC�MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6�600 nM, 75%�90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
Hybrid-LCMS/MS Method for the Quantification of Enhertu� in Rat Plasma Using Biozen� Streptavidin Magbeads
Antibody�drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu� (trastuzumab deruxtecan) as a model, we developed an LC�MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6�600 nM, 75%�90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
Hybrid-LCMS/MS Method for the Quantification of Enhertu� in Rat Plasma Using Biozen� Streptavidin Magbeads
Antibody�drug conjugates (ADCs) combine monoclonal antibodies with cytotoxic payloads for targeted therapy. Using Enhertu� (trastuzumab deruxtecan) as a model, we developed an LC�MS/MS method to quantify total antibody and conjugated payload in rat plasma. Immunocapture with streptavidin-coated magnetic beads and analysis on a Biozen Peptide XB-C18 column enabled a linear range of 0.6�600 nM, 75%�90% recovery, minimal matrix interference, and negligible carryover. This workflow provides a robust tool for ADC pharmacokinetic evaluation.
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC, HPLC Reversed Phase, Immunocapture |
| Brands: | Biozen LC |
| Separation Modes: | Reversed Phase |
| Phases: | Biozen-Peptide-XB-C18 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.