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Using dSEC-7 column for Separation and Length Determination of mRNA
In this application note, we demonstrate the use of size exclusion chromatography (SEC) with a Biozen dSEC-7 column to effectively separate in vitro transcribed (IVT) mRNA based on length. Analyzing mRNA samples ranging from 900 to 10,000 nucleotides, we observed a linear correlation between mRNA length and retention time within the 900-4000 nucleotide range, facilitating accurate length determination. This method offers a reliable analytical approach for characterizing mRNA, supporting its development in biopharmaceutical applications.

Using dSEC-7 column for Separation and Length Determination of mRNA

In this application note, we demonstrate the use of size exclusion chromatography (SEC) with a Biozen dSEC-7 column to effectively separate in vitro transcribed (IVT) mRNA based on length. Analyzing mRNA samples ranging from 900 to 10,000 nucleotides, we observed a linear correlation between mRNA length and retention time within the 900-4000 nucleotide range, facilitating accurate length determination. This method offers a reliable analytical approach for characterizing mRNA, supporting its development in biopharmaceutical applications.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Size Exclusion

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Using dSEC-7 column for Separation and Length Determination of mRNA

In this application note, we demonstrate the use of size exclusion chromatography (SEC) with a Biozen dSEC-7 column to effectively separate in vitro transcribed (IVT) mRNA based on length. Analyzing mRNA samples ranging from 900 to 10,000 nucleotides, we observed a linear correlation between mRNA length and retention time within the 900-4000 nucleotide range, facilitating accurate length determination. This method offers a reliable analytical approach for characterizing mRNA, supporting its development in biopharmaceutical applications.

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