Liquid Chromatography Separation Modes

Chromatography is widely used because it can separate many different analytes. These analytes can be separated based on various molecular characteristics, as demonstrated by the separation modes listed here.

Chiral

Separate enantiomers (identical mirror image) through the use of a chiral stationary phase

Gel Filtration (GFC)

Biomolecules or polar polymers can be separated based on their molecular weight (stokes radii)

Gel Permeation (GPC)

Efficient separations of analytes (typically ≥3 µm designed for system limitations of ≤6000 psi).

HILIC

Retain and separate highly polar analytes which cannot be retained using reversed phase

Ion Exchange

Ultra efficient separations of analytes (typically sub-2 µm particles designed for system limitations between ≥6000 psi up to 15000-20000 psi).

Ion Exclusion

Sulfonated SDVB polymers provide an environment to separate sugars, sugar alcohols and organic acids.

Normal Phase

Polar stationary phases used with non-polar mobile phase to separate hydrophobic compounds differing in the type and number of polar groups they contain.

Reversed Phase

Polar stationary phases used with non-polar mobile phase to separate hydrophobic compounds differing in the type and number of polar groups they contain.

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