Strata-X Solid Phase Extraction

Robust, Accurate Polymeric Solid Phase Extraction (SPE) Products

Capitalize on cleanliness without sacrificing recovery with Strata-X polymeric SPE sorbents for solid phase extraction. Strata-X polymeric sorbents are available in unique selectivities that have been developed to cover a diverse spectrum of analytes and simplify the method development process for fast and efficient sample preparation.

Tightly retain analytes
Remove unwanted contaminants including phospholipids, proteins, and particulates for superior chromatography
Easily cleanup large or small volumes
Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa
The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes

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Available Formats

96-Well Plates

Microelution 96-Well Plates

1, 3, and 6 mL Tubes

Giga Tubes (12-150 mL Tubes)

On-line Extraction Columns and Cartridges

Identify the SPE Retention Mechanism

Identify the Specific SPE Retention Mechanism

SPE Mechanism	Analyte Functional Group	Sorbent Functional Group	Strata-X Sorbent
Reversed Phase	R hydrocarbon aromatic	R hydrocarbon aromatic	X, XL
Ion-Exchange	NR₄+ strong RNH₃+ weak RSO₃- strong RCO₂- weak	-O₂C—weak -O₃S—strong +H₃N—weak +R₃N—strong	X-CW, XL-CW X-C X-AW, XL-AW X-A, XL-A

Select Your Particle and Pore Size

Sample Matrix	Strata-X, X-C, X-A, X-CW, X-AW	Strata-XL, XL-C, XL-A, XL-CW, XL-AW
Particle & Pore Size	33 μm, 85 Å	100 μm, 300 Å
High Concentration Samples
Small Target Analytes (< 10 kDa)
Large Target Analytes (> 10 kDa)
Large Volume Samples
Viscous Samples

Loading Capacities

Sample Matrix	Sorbent Mass	Strata-X, X-C, X-CW, X-A, X-AW	Strata-XL, XL-C, XL-CW, XL-A, XL-AW
Blood, serum, plasma	30 mg	250 µL	125 µL
Urine	30 mg	1 mL	500 µL
Filtered tissue homogenates	60 mg	100 mg	50 mg
Oral Fluid	30 mg	500 mg	250 mg

Environmental Samples	Sorbent Mass	Strata-X, X-C, X-CW, X-A, X-AW	Strata-XL, XL-C, XL-CW, XL-A, XL-AW
Water (particulate-free) drinking	200 mg	100 - 400 mL	50 - 200 mL
Water (particulate-laden) rivers, runoff, etc.	500 mg	100 - 400 mL	50 - 200 mL
Soil extracts	500 mg	100 g	50 g
Food/Plant Material	100 mg	10 mg	500 mg

Sorbent Wash and Elution Volumes*

The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.

Sorbent Mass	2 mg	10 mg	50 mg	100 mg	150 mg	200 mg	500 mg	1 g	2 g	5 g	10 g
Practical Minimum Wash and Elution Volume 4 bed volumes	200 µL	60 µL	300 µL	600 µL	900 µL	1.2 mL	3 mL	6 mL	12 mL	30 mL	60 mL
Recommended Wash and Elution Volume 8 bed volumes	50 µL	120 µL	600 µL	1.2 mL	1.8 mL	2.4 mL	6 mL	12 mL	24 mL	60 mL	120 mL

*The elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above is a guideline. An elution study should be conducted to determine the appropriate volume to use.