Optimizing Gel Permeation Chromatography (GPC) using Phenogel GPC/SEC Columns

Gel Permeation Chromatography (GPC) is principally used to separate polymers and organic molecules based on their size. An important aspect in optimizing GPC separations is column selection, especially when multiple columns are used. Mobile phase selection and column temperature are also important factors that must be considered when developing a method, as they can influence the solubility and viscosity of diluent and analyte respectively. Column, mobile phase, and temperature selection will be discussed to optimize GPC methods using Phenogel GPC/SEC columns.