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Investigation on the Impact of Using Different Mobile Phase Ratios of Acetonitrile to Methanol on Reversed Phase Phenyl Selectivity
For the greatest π-π interaction with phenyl phases, methanol is the preferred solvent over acetonitrile as the π electrons associated with the nitrile (C≡N) bond in acetonitrile compete for the π-π interaction between the phenyl phase and the analyte. However, because of the higher viscosity of methanol/water mobile phases, the addition of acetonitrile with methanol when using phenyl phases is often recommended to reduce the mobile phase viscosity and thus reduce the backpressure associated with methanol/water mixtures. In this study, we show that the addition of acetonitrile to methanol does not significantly disrupt the π-π interaction of phenyl phases, but does suppress, to some degree, the interaction as a function of the relative amount versus methanol.
Investigation on the Impact of Using Different Mobile Phase Ratios of Acetonitrile to Methanol on Reversed Phase Phenyl Selectivity
For the greatest π-π interaction with phenyl phases, methanol is the preferred solvent over acetonitrile as the π electrons associated with the nitrile (C≡N) bond in acetonitrile compete for the π-π interaction between the phenyl phase and the analyte. However, because of the higher viscosity of methanol/water mobile phases, the addition of acetonitrile with methanol when using phenyl phases is often recommended to reduce the mobile phase viscosity and thus reduce the backpressure associated with methanol/water mixtures. In this study, we show that the addition of acetonitrile to methanol does not significantly disrupt the π-π interaction of phenyl phases, but does suppress, to some degree, the interaction as a function of the relative amount versus methanol.
Document Type:
Technical Notes
Techniques:
Brands:
Separation Modes:
Reversed Phase
Investigation on the Impact of Using Different Mobile Phase Ratios of Acetonitrile to Methanol on Reversed Phase Phenyl Selectivity
For the greatest π-π interaction with phenyl phases, methanol is the preferred solvent over acetonitrile as the π electrons associated with the nitrile (C≡N) bond in acetonitrile compete for the π-π interaction between the phenyl phase and the analyte. However, because of the higher viscosity of methanol/water mobile phases, the addition of acetonitrile with methanol when using phenyl phases is often recommended to reduce the mobile phase viscosity and thus reduce the backpressure associated with methanol/water mixtures. In this study, we show that the addition of acetonitrile to methanol does not significantly disrupt the π-π interaction of phenyl phases, but does suppress, to some degree, the interaction as a function of the relative amount versus methanol.
| Target Industries: | Universities & Academia, Biopharmaceutical Analysis, Cannabis and Hemp, Chemical and Energy, Clinical Research, Environmental Testing, Food Testing. Food Safety & Food Quality Analysis., Forensic Toxicology, Pharmaceutical |
| Techniques: | HPLC, HPLC Reversed Phase |
| Brands: | Kinetex |
| Separation Modes: | Reversed Phase |
| Phases: | Kinetex Biphenyl, Kinetex C18, Kinetex F5, Kinetex Phenyl-Hexyl |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.