search
Documents
Derivatization-Free LC-MS/MS Analysis of Estrogens with Streamlined Sample Cleanup
Accurate quantitation of estrone (E1) and estradiol (E2) is critical in clinical research analysis, but their low serum concentrations, poor ionization efficiency, and structural similarity make direct LC‑MS analysis difficult. While derivatization is commonly used to boost sensitivity, advances in sample preparation now offer effective alternatives. This method showcases a highly sensitive LC‑MS/MS workflow for measuring E1 and E2 without derivatization, enabled by supported liquid extraction (SLE) for clean, efficient sample processing.
Derivatization-Free LC-MS/MS Analysis of Estrogens with Streamlined Sample Cleanup
Accurate quantitation of estrone (E1) and estradiol (E2) is critical in clinical research analysis, but their low serum concentrations, poor ionization efficiency, and structural similarity make direct LC‑MS analysis difficult. While derivatization is commonly used to boost sensitivity, advances in sample preparation now offer effective alternatives. This method showcases a highly sensitive LC‑MS/MS workflow for measuring E1 and E2 without derivatization, enabled by supported liquid extraction (SLE) for clean, efficient sample processing.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase, Supported Liquid Extraction
Phases:
Derivatization-Free LC-MS/MS Analysis of Estrogens with Streamlined Sample Cleanup
Accurate quantitation of estrone (E1) and estradiol (E2) is critical in clinical research analysis, but their low serum concentrations, poor ionization efficiency, and structural similarity make direct LC‑MS analysis difficult. While derivatization is commonly used to boost sensitivity, advances in sample preparation now offer effective alternatives. This method showcases a highly sensitive LC‑MS/MS workflow for measuring E1 and E2 without derivatization, enabled by supported liquid extraction (SLE) for clean, efficient sample processing.
| Target Industries: | Clinical Research |
| Techniques: | HPLC, HPLC Reversed Phase, Sample Preparation |
| Brands: | Kinetex, Luna Omega, Strata |
| Separation Modes: | Reversed Phase, Supported Liquid Extraction |
| Phases: | Kinetex Biphenyl, Kinetex C18, Kinetex F5, Luna Omega C18 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.