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With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:
Select a Phase to view products:
Reversed Phase
Intact C4
Intact XB-C8
Native RP-1
Native RP-5
NX-C18
Oligo
Peptide Polar-C18
Peptide PS-C18
PEPTIDE XB-C18
WIDEPORE C4
Ion Exchange
WCX
HILIC
Glycan
No Separation Mode
No Phase
Size Exclusion
dSEC-1
dSEC-2
dSEC-7
SEC-2
SEC-3
Select your ideal Biozen LC phase based on type of separation or workflow
Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!
A: UHPLC Pressure Rating
Allows for more experimental design space; room to increase flow rates for increased throughput
B: Better Controlled ID Versus PEEK Hardware
Improved column-to-column performance, providing more consistency and easier method validation
C: Biocompatible Titanium Liner
D: Improved Recovery and Peak Shape
Better column-to-column consistency, ease of use, and troubleshooting
E: Strong Stainless Steel Walls Help Prevent Movement Under Pressure
F: Biocompatible Titanium Frit
All five of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.
The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.
Meticulously controlled monosized particle technology secures incredible particle consistency that leads to improved and reliable efficiency. This innovative non-porous particle serves as the perfect backbone for complex ion-exchange chemistries.
Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogeneous porous shell is grown on a solid silica core. This highly optimized process combined with industry leading column packing technology produces highly reproducible columns that generate extremely high efficiencies and sensitivity.
Through a proprietary thermal processing series of steps, we eliminate micropores and further improve consistency, column efficiency, inertness, ruggedness, and reproducibility.
A highly engineered, non‑porous polymer particle featuring a dense, hydrophobic solid core that provides exceptional stability under native reversed‑phase conditions. Its inert, diffusion‑free architecture preserves intact, native biomolecular forms by minimizing secondary interactions while maintaining tight control over retention and elution.
The Biozen Oligo LC column brings a unique combination of improved chromatographic efficiency from its core-shell morphology and high pH stability necessary for oligonucleotide characterization. Biozen Oligo is packed in a unique bio-inert titanium hardware developed to mitigate non-specific interactions that lead to analyte loss, distortions in peak shape and carry over. Confidently maximize resolution and reproducibility throughout the most demanding of method extremes with Biozen Oligo.
Bio-inert hardware gives clear separation of the sense and anti-sense strand. View application.
Biozen 2.6 μm Oligo ( BioTi™)
Clarity 2.6 μm Oligo XT (stainless steel)
100 x 2.1 mm
A: 4 mM Triethylamine in Water + 12.5 mM Hexafluoro 2 propanol,
B: 4 mM Triethylamine in Methanol + 12.5 mM Hexafluoro 2 propanol
vs
Non-specific interactions were observed using stainless steel hardware resulting in bimodal peak for late eluting anti-sense strand.
0.3 mL/min
55 °C
TOF-MS
Shimadzu® LC 20A Prominence®
SCIEX® TripleTOF ® 6600
0
2
16
16
16.1
20
20.1
5
5
30
95
95
5
Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed two Biozen Peptide columns to offer uniquely different selectivity profiles for these types of bioseparations. Each allows for fast and effective elution windows by utilizing either high efficiency core-shell or thermally modified fully porous particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.
Column: |
Biozen 1.6 µm Peptide PS-C18 Biozen 2.6 µm Peptide XB-C18 |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.3 mL/min |
Temperature: | 40 °C |
Detection: | QTOF (SCIEX® X500B) |
Mobile Phase: | A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile |
Part No.: |
Gradient : Time (min) | % B |
| 0 | 1 |
| 0.5 | 1 |
| 50 | 50 |
| 56 | 95 |
With protein aggregate often at very low levels (<0.1% by peak area compared to monomer) and fragment separation a requirement, adequate resolution and peak shape have become even more crucial method outcomes. To address this need, the robust set of Biozen SEC columns were developed with a combination of UHPLC efficiency and higher sensitivity, to drive resolution and identification of even lower level targets.
View Biozen dSEC-7 Brochure
View Biozen dSEC-2 Brochure
Column: | Biozen 3 µm dSEC-7 |
Dimensions | 150 x 4.6 mm |
Flow Rate: | 350 µL/min (Isocratic) |
Temperature: | 25 °C |
Detection: | FLR - Ex 280 nm, Em 350 nmSampling Rate: 40 Hz |
Mobile Phase: | 200 Potassium Phosphate +250 mM KCl, pH 6.2 |
Mobile Phase: | 20 mM Sodium Phosphate, pH 6.6 + 350 mM Potassium Chloride |
Part No.: | |
Part No.: | 00H-4787-E0 |
Injection Volume: | 0.5 µL, 1 µL, 2 µL |
Instrument: | Waters® ACQUITY® H-Class |
Sample
Column: | Biozen 1.8 µm dSEC-2, 200 Å |
Dimensions | 300 x 4.6 mm |
Part No.: | |
Mobile Phase: | 200 Potassium Phosphate +250 mM KCl, pH 6.2 |
Flow Rate: | 0.35 mL/min |
Injection Volume: | 10 µL |
Temperature: | 25 °C |
Detection: | UV @ 280 nm |
Sample: | Various, 10 mg/mL |
.
Biozen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly tuned and controlled manufacturing process, Biozen WCX HPLC columns affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware. View Application Note.
Column: | Biozen 6 μm WCX |
Dimensions | 250 x 4.6 mm |
Flow Rate: | 1 mL/min |
Temperature: | 30 °C |
Detection: | UV @ 280 nm |
Mobile Phase: | A: 20 mM MES (pH 5.6), B: 20 mM MES + 300 mM NaCl (pH 5.6) |
Part No.: |
Gradient : Time (min) | % B |
| 0 | 15 |
| 1 | 15 |
| 31 | 45 |
| 31.1 | 100 |
| 34 | 100 |
| 35 | 15 |
Column: | Biozen 6 μm WCX |
Dimensions | 250 x 4.6 mm |
Flow Rate: | 1 mL/min |
Temperature: | 30 °C |
Detection: | UV @ 280 nm |
Mobile Phase: | A: CX -1 (pH 5.6) pH Gradient Buffer*, B: CX -1 (pH 10.2) pH Gradient Buffer* |
Part No.: |
Gradient : Time (min) | % B |
| 0 | 0 |
| 1 | 0 |
| 21 | 100 |
| 23 | 100 |
| 24 | 0 |
Sample:
Trastuzumab
Impurity profiling and characterization of intact biologics and fragments is a challenging undertaking because of the need to identify very small differences between variants. Both Biozen Intact columns. contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target mAb HC/LC, Fc/Fab, or isoforms.
Column: | Biozen 3.6 µm Intact XB-C8 |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.5 mL/min |
Temperature: |
⬜70 °C 🟦80 °C ⬛90 °C |
Detection: | UV @ 280 nm |
Mobile Phase: | A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile |
Part No.: |
Gradient : Time (min) | % B |
| 0 | 20 |
| 1 | 20 |
| 13 | 25 |
Sample:
Trastuzumab
Column: | Biozen 3.6 µm Intact XB-C8 |
Dimensions | 150 x 2.1 mm |
Part No.: | |
Mobile Phase: | A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile |
Flow Rate: | 0.5 mL/min |
Temperature: | 80 ° |
Detection: | UV @ 280 nm |
Gradient : Time (min) | % B |
| 0 | 20 |
| 1 | 20 |
| 13 | 60 |
Sample:
Infliximab F(ab)2
Column: | Biozen 3.6 µm Intact XB-C8 |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.5 mL/min |
Temperature: | 80 ° |
Detection: | UV @ 280 nm |
Mobile Phase: | A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile |
Part No.: |
Gradient : Time (min) | % B |
| 0 | 20 |
| 1 | 20 |
| 13 | 45 |
Sample:
Cetuximab
Intact mass can give indications not only of relative abundance of glycoforms , but also stability as degraded mAbs will not give good charge envelope by ESI-MS. Intact Mass with a high resolution MS to identify PTMs, especially relative abundance of glycoforms , combines extremely well with the fast run times and tight peak shapes provided by the Biozen Intact XB-C8 and Biozen WidePore C4.
Column: | Biozen 3.6 µm Intact XB-C8 |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.3 mL/min |
Temperature: | 90 ° |
Detection: | QTOF (SCIEX X500B) |
Mobile Phase: | A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile / Isopropyl alcohol (50:50 |
Part No.: |
Gradient : Time (min) | % B2 |
| 2.5 | 0 |
| 10 | 65 |
| 110.1 | 95 |
Sample:
Trastuzumab
With a direct effect on efficacy and safety, conjugation for each ADC must be well understood. The Biozen Intact XB-C8 provides an excellent vehicle for determining drug load distribution and DAR for ADCs. Its large pore size allows intact ADCs to interact with a moderately retentive stationary phase while the core-shell particle supplies increased efficiency to deliver the required resolution between ADC species with differing drug loads.
When quantitating signature peptides from biological matrices, you need sharp peak shape and sufficient retention of hydrophilic peptides to prevent any signal loss from matrix suppression regions. Both Biozen Peptide columns were developed to deliver excellent selectivity for even closely related peptides. Additionally, they build on this body of valuable characteristics with unique ways of delivering sharper peak shape for basic peptides; Biozen Peptide XB-C18 blocks secondary surface interactions via isobutyl side chains, while the Biozen Peptide PS-C18 contains a positively charged weak base that repels other basic species.
Column: | Biozen 3 µm Peptide PS-C18 |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.5 mL/min |
Flow Rate: | 1.85 mL/min |
Temperature: | 22 °C |
Detection: | MS/MS |
Mobile Phase: | A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile |
Part No.: | |
LC System: | ExionLC™ AD HPLC |
Detector: | SCIEX QTRAP® 5500 |
Gradient : Time (min) | % B |
| 0 | 3 |
| 1 | 3 |
| 4.5 | 25 |
Sample:
As noted
The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the Biozen Glycan excels with increased polar retention and selectivity.
Column: | Biozen 2.6 µm Glycan |
Dimensions | 150 x 2.1 mm |
Flow Rate: | 0.5 mL/min |
Temperature: | 50 °C |
Detection: | FLD ex/em 285/345 nm |
Mobile Phase: | A: 100 mM Ammonium Formate, pH 4.5, B: Acetonitrile |
Part No.: | 00F-4773-AN |
Gradient : Time (min) | % B |
| 0 | 78 |
| 10 | 74.5 |
| 24 | 72 |
| 38.5 | 55.9 |
| 38.6 | 40 |
| 40.6 | 40 |
| 40.7 | 78 |
| 48 | 78 |
Sample:
As noted
90Å low pore volume silica couples with hydrophilic diol-type surface chemistry to mitigate unwanted silica surface interactions.
Recommended Use
Analysis of peptides, protein fragments, and small biologics with reduced analysis times and improved column lifetimes.
A low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples
Recommended Use
Analysis of monoclonal antibodies, biosimilars and other biomolecules by using specific molecular weights with improved column lifetimes and recovery
Particle Size
3 µm, 1.8 µm
Large pore silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry for the separation and quantitation of AAV aggregate analysis.
Recommended Use
Aggregate analysis of AAVs and other large biomolecules using specific molecular weights that conserves sample consumption, facilitates faster run times, and maximizes resolution.
Core-shell particle bonded with amide polyol
Recommended Use
High efficiency and selectivity for released glycans
Particle Size
2.6 µm
A core-shell particle bonded with a C4 ligand
Recommended Use
Intact and subunit analysis of large biomolecules, such as proteins and mAbs
Particle Size
3.6 µm
Large pore core-shell particle bonded with C8
Recommended Use
Fast separation of intact and subunit separation with hydrophobic selectivity
Particle Size
3.6 µm
Recommended Use
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Recommended Use
—
Recommended Use
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High pH Fractionation Column
Recommended Use
Minimizes nonspecific adsorption resulting in increased confidence in your peptide identifications
Particle Size
3 µm
An organo-silica core-shell bonded with a C18 stationary phasefor performance gains (speed, sensitivity, resolution) plus the high pH ruggedness necessary for oligonucleotide separations
Recommended Use
Analytical characterization of oligonucleotides
Particle Size
2.6 µm, 1.7 µm
a core-shell particle bonded with a polar c18 ligand
Recommended Use
Enhanced selectivity/retention for polar analytes in a nano flow format
