Axia Preparative LC Columns
Axia Preparative LC Columns help you develop and scale purification methods with more predictable results. Patented self-packing technology creates stable beds that reduce wall effects and downtime. You can scale up throughput without changing particle size while keeping selectivity. Broad media choices and dimensions from 10 mm to 300 mm ID support many prep LC systems and workflows. Custom packing services, brochure downloads, and quote options give you practical support for method development and scale-up.
- Axia Preparative LC Columns help you make purification method development and scale-up more predictable and reproducible.
- Axia columns work with major preparative LC systems and come in dimensions from 10 mm to 300 mm ID.
- The patented self-packing process creates stable packed beds that reduce wall effects and downtime.
- You can scale up throughput without changing particle size while maintaining selectivity.
- A wide range of phases, dimensions, and media options helps simplify prep method development and scale-up.
Guaranteed, Longest Lasting Preparative HPLC and SFC Column
Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative HPLC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.
Overview
Phenomenex Axia Preparative LC Column Portfolio
Phenomenex offers a diverse portfolio of preparative LC columns that are engineered to meet the wide range of purification challenges faced. Each column brand is designed with unique selectivity, scalability, and loading capacity in mind, ensuring reliable performance across small-scale method development and large-scale production. Explore our most popular HPLC brands.
Axia Single Axial Compression Step
Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. The ideal prep HPLC column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time.
Axia™ packed columns produce uniform media bed with intact particles
The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed HPLC prep columns.
Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles
Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes.
Unmatched Column Reproducibility
The automated Axia™ packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns.
Compare Lifetime
Comparative separations may not be representative of all applications. ‡Same linear velocity
Conditions for all columns:
Columns: | Luna 5 µm C18(2) Axia Packed, Waters® XTerra® 5 µm Prep MS C18 OBD™ |
Dimension: | 50 x 21.2 mm (Luna),50 x 19 mm (XTerra) |
Flow Rate: | 30 mL/min (Luna), 24 mL/min (XTerra)‡ |
Temperature: | Ambient |
Detection: | UV @ 254 nm |
Mobile Phase: | A: 0.5 % TFA in Water, B: 0.5 % TFA in Acetonitrile Formic Acid |
Gradient: | Linear 95:5 (A/B) to 5:95 (A/B), over 7 min, hold 3 min |
Injection Volume: | 500 μL |
Sample
- Triprolidine 1.6 mg
- Methacycline 16 mg
- Amitriptyline 5.25 mg
Seamless Scalability to Increase Sample Load
Increase sample load without increasing your flow rate by using a longer column. With Axia technology, each preparative column is optimized for:
- Analytical-like efficiency
- Long column lifetime
- High sample load with high surface area media such as Kinetex, Aeris, Gemini, Luna, Luna Omega, or Synergi
Condition:
Flow Rate: | 30 mL/min |
Gradient: | 10 min on 100 mm length column 25 min on, 250 mm length column 5 % to 95 %, Acetonitrile, 0.5 % TFA |
Boost Throughput with Larger Columns
For maximizing load without increasing the run time, consider scaling up to a larger column ID. Axia packed columns provide the three important benefits you need.
- Reproducible performance across all column diameters
- Increased throughput without sacrificing purity
- High efficiency from analytical to preparative
Analytical 50 x 4.6 mm
Dimension: | 50 x 4.6 mm |
Flow Rate | 1.5 mL/min |
Sample Load: | 0.1 mg in 21 µL DMSO |
Axia PREP 50 x 21.2 mm
Dimension: | 50 x 21.2 mm |
Flow Rate | 30 mL/min |
Sample Load: | 2.1 mg in 420 µL DMSO |
Axia PREP 50 x 30 mm
Dimension: | 50 x 30 mm |
Flow Rate | 60 mL/min |
Sample Load: | 4.2 mg in 840 µL DMSO |
Axia PREP 50 x 50 mm
Dimension: | 50 x 50 mm |
Flow Rate | 150 mL/min |
Sample Load: | 10.5 mg in 2100 µL DMSO |
Axia Technology Outperforms Traditional Packing Processes!
Because of the constant pressure placed on the integrated packing piston, Axia packed columns possess the dynamic capability of maintaining a consistent, homogeneous media bed. This results in superior column performance no matter which media selectivity you choose.
Warfarin Chiral Purification in Normal Phase Mode
Column: | |
Dimension: | 150 x 4.6 mm |
Flow Rate: | 1 mL/min |
Temperature: | Ambient |
Mobile Phase: | Hexane / Ethanol (75:25) |
Injection Volume: | 100 µL |
Conditions for both PREP columns:
Column: | |
Dimension: | 150 x 21.2 mm |
Flow Rate: | 20 mL/min |
Temperature: | Ambient |
Mobile Phase: | Hexane / Ethanol (75:25) |
Injection Volume: | 20 mL |
42 % Increase in Axia Efficiency
| Column (mm) | Analytical 150 x 4.6 | Standard 150 x 21.2 | Axia 150 x 21.2 |
|---|---|---|---|
| Mass Loaded (mg) | 2 | 40 | 40 |
| Resolution* | 1.5 | 2.85 | 3.72 |
| Plates (N) | 117 | 535 | 760 |
Axia column and packing technology is patented by Phenomenex. U.S. Patent No. 7, 674, 383
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