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Product Information
Phase Detail
Rezex RNM-Carbohydrate Na<sup>+</sup> Ion Exclusion HPLC Columns<br class="t-last-br" />
Rezex RNM-Carbohydrate Na+ Ion Exclusion HPLC Columns is packed with 8% cross-linked sodium ion exclusion media

Rezex RNM-Carbohydrate Na+ Ion Exclusion HPLC Columns

  • 8% cross-linked sodium
  • For the analysis of matrices containing high concentrations of inorganic sodium (e.g. molasses)
  • Analysis of mono-, di-, and tri- saccharides
  • Easy to regenerate the original ionic strength
  • USP L58

Particle Size
8
Recommended Use
Analysis of mono-, di-,and tri- saccharides and for matrices which contain high concentration of inorganic sodium

Cross Reference Guide

Phase Description Applications Bio-Rad®
Aminex®
Supelco®
Supelcogel
Waters®
SugarPak
Transgenomic®
CARBOSep
Sepax®
Carbomi®
RNM-Carbohydrate
(USP L58 packing)
8 %
cross-linked
SODIUM
  • Analysis of mono-, di-, and tri- saccharides
  • For matrices which contain high concentration of inorganic sodium (i.e. molasses)
HPX-87N
125-0143
N/A N/A N/A Carbomix Na

Retention Times for Some Carbohydrates and Sugar Alcohols

Counter Ion Analyte RNM Na+
Adonitol (Ribitol) 11.10
D-Altrose 11.45
D-(-)-Arabinose 12.65
D-(+)-Cellobiose 8.49
D-(+)-Digitoxose 11.39
Dulcitol 11.10
Meso-Erythritol 11.78
D-(-)-Fructose 11.76
L-(-)-Fucose 12.30
D-(+)-Galactose 11.47
Gentiobiose 8.40
D-(+)-Glucose 10.71
Inositol 12.14
Isomaltose 8.76
Lactose 8.78
Lactulose 9.23
D-Lyxose 11.98

Counter Ion Analyte RNM Na+
D-Maltose 8.75
Maltotriose 7.94
Maltulose 8.82
D-Mannitol 10.80
D-(+)-Mannose 11.54
Melibiose 8.82
D-(+)-Melezitose 7.66
D-(+)-Raffinose 7.76
L-(+)-Rhamnose 11.00
D-(-)-Ribose 14.34
Salicin 17.36
D-Sorbitol 11.39
Stachyose 7.30
Sucrose 8.65
Trehalose 8.49
Xylitol 12.16
D-(+)-Xylose 11.68

Select Applications on Rezex RNM-Carbohydrate Columns

Carbohydrates

Column:
Rezex RNM-Carbohydrate
Dimension:
300 x 7.8 mm
Part No.:
00H-0136-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
RI
Sample:
1. Stachyose
2. Cellobiose
3. Glucose
4. Fructose
5. Arabinose
6. Ribose
7. Salicin
(1 % solution of each)

Applications (partial list)

Call us for assistance in selecting the right column for your needs!

Compound App ID No.
Arabinose 5510
D-Cellobiose 5510
Fructose 5510
Glucose 5510

Compound App ID No.
Ribose 5510
Salicin 5510
Stachyose 5510

Specifications and Operating Recommendations

Rezex RNM-Carbohydrate
Part No. 00H-0136-K0
Ionic Form Sodium
Standard Dimensions 300 x 7.8 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8 %
Particle Size (μm) 8
Min. Efficiency (p/m) based on last peak 30,000
Typical Pressure (psi @ Testing Flow Rate) 170
Max. Pressure (psi @ Max Flow Rate) 1,000
Max. Flow Rate (mL/min) 1.0
Max. Temperature (°C) 85
Typical Mobile Phase Water
pH Range Neutral
Guard Column Part 03B-0136-K0


Cleaning, Regeneration and Storage 
Organic Modifiers (Max) 5 % Methanol, IPA, EtOH
Inorganic Modifiers 5 % Sodium Salts
Avoid Acids, Bases, Non-Sodium Salts or Metal Ions, > 30 % Acetonitrile
Cleaning Solvent 100 % Water
Flow Rate(mL/min) 0.4
Temperature (°C) 85
Duration (hrs) 12
Regeneration Solvent 0.1 M NaNO3
Flow Rate (mL/min) 0.2
Duration (hrs) 4-16
Ship/Storage Solvent Water