Rezex RNM-Carbohydrate Na+ Ion Exclusion HPLC Columns

DNA, RNA, miRNA, siRNA, phosphorothioates, LNA, single stranded, duplexed, and encapsulated oligonucleotides. As long as there is a phosphodiester or phosphorothioate backbone the extraction protocol should provide excellent clean-up and recovery

The mechanism for sugar separations is a mixture of size exclusion and affinity of the sugars towards the metal ion associated with the phase. The sugars form complexes with the metal ion, the strength of which dictate their order of elution from the column.

Proper care of an HPLC is critically important for lifetime of a Rezex column. Removing organic solvent from the fluid lines as well as the needle wash prevents the column from being subjected to a slug of high organic solvent that can kill a column. Similarly, metal bound Rezex columns (RCM, RPM, RAM, RSO, and RNM) are sensitive to acidic conditions and buffer salts that used for other HPLC separation. Finally, microbial contamination of mobile phase and the HPLC are a constant concern for using Ion Exclusion columns. Mobile phase should be changed frequently and the system should flushed from time to time with organic or dilute nitric acid (of course with the column removed) to reduce microbial contamination (see your HPLC manufacturers guide to use their preferred cleaning procedure).

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