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    Biozen Biologics LC Columns

    Biozen LC Columns support biomolecule analysis across SEC, Glycan, Intact, Peptide, dSEC-2, and mAb workflows. The portfolio cuts secondary interactions and sample adsorption. This supports accurate protein aggregate analysis. It also supports fast glycan profiling and peptide mapping. Depending on the column, pH stability ranges from 2 to 8 or 1 to 12. Biozen also improves recovery, peak shape, sensitivity, and throughput for intact proteins, mRNA, oligonucleotides, and mAbs. You get better data quality, broader workflow options, and faster method development.

    • Biozen LC Columns cover SEC, Glycan, Intact, Peptide, dSEC-2, and mAb workflows for biomolecule analysis.
    • Biozen SEC columns cut secondary interactions and sample adsorption to support accurate protein aggregate analysis.
    • Biozen Glycan and Peptide columns support fast separations with pH stability ranges of 2 to 8 and 1 to 12.
    • Biozen Intact, dSEC-2, and mAb columns improve recovery, peak shape, sensitivity, and throughput for complex biomolecules.
    • The portfolio helps labs get better data quality, broader workflow options, and faster method development.
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    Explore Biozen LC Portfolio

    With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:

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    Select a Phase to view products:

    Reversed Phase

    Intact C4

    Intact XB-C8

    Native RP-1

    Native RP-5

    NX-C18

    Oligo

    Peptide Polar-C18

    Peptide PS-C18

    PEPTIDE XB-C18

    WIDEPORE C4

    Ion Exchange

    WCX

    HILIC

    Glycan

    No Separation Mode

    No Phase

    Size Exclusion

    dSEC-1

    dSEC-2

    dSEC-7

    SEC-2

    SEC-3

    Product Finder

    Select your ideal Biozen LC phase based on type of separation or workflow

    Overview

    Biocompatible Flow Path with BioTi Hardware

    Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!

    A: UHPLC Pressure Rating

    Allows for more experimental design space; room to increase flow rates for increased throughput

    B: Better Controlled ID Versus PEEK Hardware

    Improved column-to-column performance, providing more consistency and easier method validation

    C: Biocompatible Titanium Liner

    D: Improved Recovery and Peak Shape

    Better column-to-column consistency, ease of use, and troubleshooting

    E: Strong Stainless Steel Walls Help Prevent Movement Under Pressure

    F: Biocompatible Titanium Frit

    5 Advanced Particle Platforms

    All five of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.

    • Pore Controlled Technology

      The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.

    • Monosized Polymeric Non-Porous

      Meticulously controlled monosized particle technology secures incredible particle consistency that leads to improved and reliable efficiency. This innovative non-porous particle serves as the perfect backbone for complex ion-exchange chemistries.

    • Core-Shell Technology

      Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogeneous porous shell is grown on a solid silica core. This highly optimized process combined with industry leading column packing technology produces highly reproducible columns that generate extremely high efficiencies and sensitivity.

    • Thermally Modified Fully Porous

      Through a proprietary thermal processing series of steps, we eliminate micropores and further improve consistency, column efficiency, inertness, ruggedness, and reproducibility.

    • Solid Core Polymer Technology

      A highly engineered, non‑porous polymer particle featuring a dense, hydrophobic solid core that provides exceptional stability under native reversed‑phase conditions. Its inert, diffusion‑free architecture preserves intact, native biomolecular forms by minimizing secondary interactions while maintaining tight control over retention and elution.

    15 Particle Chemistries

    • SEC

      Biozen dSEC-1
      1.6 µm and 3 µm

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    • SEC

      Biozen dSEC-2
      1.8 µm and 3 µm

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    • SEC

      Biozen SEC-2
      1.8 µm

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    • SEC

      Biozen SEC-3
      1.8 µm

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    • SEC

      Biozen dSEC-7
      3 µm

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    • Intact

      Biozen Intact XB C8
      3.6 µm

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    • Intact

      Biozen WidePore C4
      2.6 µm

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    • Glycan

      Biozen Glycan
      2.6 µm

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    • Oligonucleotides

      Biozen Oligo
      1.7 µm and 2.6 µm

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    • Peptide

      Biozen Peptide XB-C18
      1.7 µm and 2.6 µm

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    • Peptide

      Biozen Peptide PS-C18
      1.6 µm and 3 µm

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    • Peptide

      Biozen Peptide Polar-C18
      1.6 µm and 3 µm

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    • Ion-Exchange

      Biozen WCX
      6 µm

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    • Native

      Biozen Native RP-1
      3 µm

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    • Native

      Biozen Native RP-5
      3 µm

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    Workflow

    Oligonucleotide Analysis

    The Biozen Oligo LC column brings a unique combination of improved chromatographic efficiency from its core-shell morphology and high pH stability necessary for oligonucleotide characterization. Biozen Oligo is packed in a unique bio-inert titanium hardware developed to mitigate non-specific interactions that lead to analyte loss, distortions in peak shape and carry over. Confidently maximize resolution and reproducibility throughout the most demanding of method extremes with Biozen Oligo.

    LC-MS Analysis of siRNA using BioTi UHPLC Hardware

    Bio-inert hardware gives clear separation of the sense and anti-sense strand. View application.

    Columns:

    Biozen 2.6 μm Oligo ( BioTi™)

    Clarity 2.6 μm Oligo XT (stainless steel)

    Dimensions:

    100 x 2.1 mm

    Mobile Phase:

    A: 4 mM Triethylamine in Water + 12.5 mM Hexafluoro 2 propanol,

    B: 4 mM Triethylamine in Methanol + 12.5 mM Hexafluoro 2 propanol

    Part Number:

    00D-4790-AN (Biozen)
    00D-4746-AN (Clarity)

    vs

    LC-MS Analysis of siRNA using Stainless Steel UHPLC Hardware

    Non-specific interactions were observed using stainless steel hardware resulting in bimodal peak for late eluting anti-sense strand.

    Flow Rate:

    0.3 mL/min

    Temperature:

    55 °C

    Detection:

    TOF-MS

    Instrument:

    Shimadzu® LC 20A Prominence®

    Detector:

    SCIEX® TripleTOF ® 6600

    Gradient:
    Time (min)

    0

    2

    16

    16

    16.1

    20

    20.1

    % B

    5

    5

    30

    95

    95

    5

    Peptide Mapping

    Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed two Biozen Peptide columns to offer uniquely different selectivity profiles for these types of bioseparations. Each allows for fast and effective elution windows by utilizing either high efficiency core-shell or thermally modified fully porous particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.

    Trastuzumab Biosimilar Peptide Map

    Infliximab Biosimilar Peptide Map

    Conditions for both columns:

    Column:

    Biozen 1.6 µm Peptide PS-C18

    Biozen 2.6 µm Peptide XB-C18

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.3 mL/min

    Temperature:

    		40 °C

    Detection:

    		QTOF (SCIEX®  X500B)

    Mobile Phase:

    		A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile

    Part No.:

    00F-4770-AN
    00F-4768-AN

    Gradient : Time (min)

    % B

    01
    0.51
    5050
    5695

    Aggregate Analysis

    With protein aggregate often at very low levels (<0.1% by peak area compared to monomer) and fragment separation a requirement, adequate resolution and peak shape have become even more crucial method outcomes. To address this need, the robust set of Biozen SEC columns were developed with a combination of UHPLC efficiency and higher sensitivity, to drive resolution and identification of even lower level targets.
    View Biozen dSEC-7 Brochure
    View Biozen dSEC-2 Brochure

    Column:

    		Biozen 3 µm dSEC-7

    Dimensions

    		150 x 4.6 mm

    Flow Rate:

    		350 µL/min (Isocratic)

    Temperature:

    		25 °C

    Detection:

    		FLR - Ex 280 nm, Em 350 nmSampling Rate: 40 Hz

    Mobile Phase:

    		200 Potassium Phosphate +250 mM KCl, pH 6.2

    Mobile Phase:

    		20 mM Sodium Phosphate, pH 6.6 + 350 mM Potassium Chloride

    Part No.:

    00F-4789-E0

    Part No.:

    		00H-4787-E0

    Injection Volume:

    		0.5 µL, 1 µL, 2 µL

    Instrument:

    		Waters® ACQUITY® H-Class

    Sample

    1. AAV2-CAG-GFP, 2E13 vg/mL
    2. AAV5-CMV-GFP, 2E13 vg/mL
    3. AAV8-CMV-GFP, 2E13 vg/mL
    4. AAV9-CMV-GFP, 2E13 vg/mL

    Column:

    		Biozen 1.8 µm dSEC-2, 200  Å

    Dimensions

    		300 x 4.6 mm

    Part No.:

    00H-4787-E0

    Mobile Phase:

    		200 Potassium Phosphate +250 mM KCl, pH 6.2

    Flow Rate:

    		0.35 mL/min

    Injection Volume:

    		10 µL

    Temperature:

    		25 °C

    Detection:

    		UV @ 280 nm

    Sample:

    		Various, 10 mg/mL

    .

    Charge Variant Analysis

    Biozen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly tuned and controlled manufacturing process, Biozen WCX HPLC columns affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware. View Application Note.

    Trastuzumab (MES Salt Gradient)

    Column:

    		Biozen 6 μm WCX

    Dimensions

    		250 x 4.6 mm

    Flow Rate:

    		1 mL/min

    Temperature:

    		30 °C

    Detection:

    		UV @ 280 nm

    Mobile Phase:

    		A: 20 mM MES (pH 5.6), B: 20 mM MES + 300 mM NaCl (pH 5.6)

    Part No.:

    00G-4777-E0

    Gradient : Time (min)

    % B

    015
    115
    3145
    31.1100
    34100
    3515

    Column:

    		Biozen 6 μm WCX

    Dimensions

    		250 x 4.6 mm

    Flow Rate:

    		1 mL/min

    Temperature:

    		30 °C

    Detection:

    		UV @ 280 nm

    Mobile Phase:

    		A: CX -1 (pH 5.6) pH Gradient Buffer*, B: CX -1 (pH 10.2) pH Gradient Buffer*

    Part No.:

    00G-4777-E0

    Gradient : Time (min)

    % B

    00
    10
    21100
    23100
    240

    Sample:
    Trastuzumab

    Intact and Subunit Analysis

    Impurity profiling and characterization of intact biologics and fragments is a challenging undertaking because of the need to identify very small differences between variants. Both Biozen Intact columns. contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target mAb HC/LC, Fc/Fab, or isoforms.

    Intact Trastuzumab at 70, 80, and 90 °C

    Column:

    		Biozen 3.6 µm Intact XB-C8

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.5 mL/min

    Temperature:

    ⬜70 °C

    🟦80 °C

    ⬛90 °C

    Detection:

    		UV @ 280 nm

    Mobile Phase:

    		A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile

    Part No.:

    00F-4766-AN

    Gradient : Time (min)

    % B

    020
    120
    1325

    Sample:
    Trastuzumab

    Infliximab F(ab)2

    Column:

    		Biozen 3.6 µm Intact XB-C8

    Dimensions

    		150 x 2.1 mm

    Part No.:

    00F-4766-AN

    Mobile Phase:

    		A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile

    Flow Rate:

    		0.5 mL/min

    Temperature:

    		80 °

    Detection:

    		UV @ 280 nm

    Gradient : Time (min)

    % B

    020
    120
    1360

    Sample:
    Infliximab F(ab)2

    Cetuximab

    Column:

    		Biozen 3.6 µm Intact XB-C8

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.5 mL/min

    Temperature:

    		80 °

    Detection:

    		UV @ 280 nm

    Mobile Phase:

    		A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile

    Part No.:

    00F-4766-AN

    Gradient : Time (min)

    % B

    020
    120
    1345

    Sample:
    Cetuximab

    Intact Mass Analysis

    Intact mass can give indications not only of relative abundance of glycoforms , but also stability as degraded mAbs will not give good charge envelope by ESI-MS. Intact Mass with a high resolution MS to identify PTMs, especially relative abundance of glycoforms , combines extremely well with the fast run times and tight peak shapes provided by the Biozen Intact XB-C8 and Biozen WidePore C4.

    Intact Mass of Trastuzumab Biosimilar using a Biozen Intact XB-C8 and SCIEX® X500B

    Column:

    		Biozen 3.6 µm Intact XB-C8

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.3 mL/min

    Temperature:

    		90 °

    Detection:

    		QTOF (SCIEX X500B)

    Mobile Phase:

    		A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile / Isopropyl alcohol (50:50

    Part No.:

    00F-4766-AN

    Gradient : Time (min)

    % B2

    2.50
    1065
    110.195

    Sample:
    Trastuzumab

    Drug Antibody Ratio (DAR)

    With a direct effect on efficacy and safety, conjugation for each ADC must be well understood. The Biozen Intact XB-C8 provides an excellent vehicle for determining drug load distribution and DAR for ADCs. Its large pore size allows intact ADCs to interact with a moderately retentive stationary phase while the core-shell particle supplies increased efficiency to deliver the required resolution between ADC species with differing drug loads.

    Herceptin—vcMMAE using Biozen 3.6 µm Intact XB-C8

    Herceptin—mcMMAF using Biozen 3.6 µm Intact XB-C8

    Peptide Quantitation

    When quantitating signature peptides from biological matrices, you need sharp peak shape and sufficient retention of hydrophilic peptides to prevent any signal loss from matrix suppression regions. Both Biozen Peptide columns were developed to deliver excellent selectivity for even closely related peptides. Additionally, they build on this body of valuable characteristics with unique ways of delivering sharper peak shape for basic peptides; Biozen Peptide XB-C18 blocks secondary surface interactions via isobutyl side chains, while the Biozen Peptide PS-C18 contains a positively charged weak base that repels other basic species.

    Column:

    		Biozen 3 µm Peptide PS-C18

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.5 mL/min

    Flow Rate:

    		1.85 mL/min

    Temperature:

    		22 °C

    Detection:

    		MS/MS

    Mobile Phase:

    		A: 0.1 % Formic Acid in Water, B: 0.1 % Formic Acid in Acetonitrile

    Part No.:

    00B-4771-AN

    LC System:

    		ExionLC™ AD HPLC

    Detector:

    		SCIEX QTRAP® 5500

    Gradient : Time (min)

    % B

    03
    13
    4.525

    Sample:
    As noted

    Glycan Analysis

    The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the Biozen Glycan excels with increased polar retention and selectivity.

    Intact Mass of Trastuzumab Biosimilar using a Biozen Intact XB-C8 and SCIEX® X500B

    Column:

    		Biozen 2.6 µm Glycan

    Dimensions

    		150 x 2.1 mm

    Flow Rate:

    		0.5 mL/min

    Temperature:

    		50 °C

    Detection:

    		FLD ex/em 285/345 nm

    Mobile Phase:

    		A: 100 mM Ammonium Formate,  pH 4.5, B: Acetonitrile

    Part No.:

    		00F-4773-AN

    Gradient : Time (min)

    % B

    078
    1074.5
    2472
    38.555.9
    38.640
    40.640
    40.778
    4878

    Sample:
    As noted

    Oligonucleotide Solid Phase Extraction (SPE)